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Product Info Summary
| SKU: | M11223 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-TRIM Purified TRAT1 Monoclonal Antibody
SKU/Catalog Number
M11223
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TRIM Purified TRAT1 Monoclonal Antibody (Catalog# M11223). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-TRIM Purified TRAT1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11223)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
TRIM-04
Isotype
Mouse IgG2a
Immunogen
Recombinant intracellular domain (aa 29-186) of human TRIM. The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M11223 is reactive to TRAT1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
21.2 kDa
Background of TRAT1
TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11223 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 µg/ml, intracellular staining.
Western blotting: Non-reducing conditions.
Validation Images & Assay Conditions
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Western blotting analysis of human Jurkat T cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by mAb TRIM-04 and goat anti-mouse IgG (H+L)-HRP conjugate.
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Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-TRIM (TRIM-04) purified antibody (concentration in sample 1 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).
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Separation of human CD3 positive TRIM positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-TRIM (TRIM-04) purified antibody (concentration in sample 1 µg/ml, GAM APC).
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Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-TRIM (TRIM-04) purified antibody (concentration in sample 1 µg/ml, GAM APC).
Specific Publications For Anti-TRIM Purified TRAT1 Monoclonal Antibody (M11223)
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