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- Table of Contents
Trim16 antibody (A09514-4) performs well in ELISA, IF, IHC, ICC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of TRIM16 using anti-TRIM16 antibody (A09514-4).
TRIM16 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TRIM16 Antibody (A09514-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
TRIM16 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-TRIM16 Antibody (A09514-4) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-TRIM16 Antibody Picoband®
Trim16 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | ELISA, IF, IHC, ICC, WB |
Tested Applications
Trim16 antibody was positively detected by WB in:
rat testis tissue, rat C6 whole cell, mouse NIH/3T3 whole cell, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Trim16 antibody was positively detected by IHC in:
mouse intestine tissue, rat intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Trim16 antibody was positively detected by ICC/IF in:
HEPA1-6 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Mouse
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Product Information
Product Name
Anti-TRIM16 Antibody Picoband®
View all TRIM16 Antibodies
View all TRIM16 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse TRIM16 recombinant protein (Position: D65-K210).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
63 kDa
Calculated Molecular Weight
62.9 kDa
Background of Trim16
Tripartite motif-containing protein 16 is a protein that in humans is encoded by the TRIM16 gene. This gene was identified as an estrogen and anti-estrogen regulated gene in epithelial cells stably expressing estrogen receptor. The protein encoded by this gene contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. The proteins of this family have been reported to be involved in a variety of biological processes including cell growth, differentiation and pathogenesis. Expression of this gene was detected in most tissues. Its function, however, has not yet been determined.
Cite This Product
Anti-TRIM16 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09514-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
97%
High Homology
Rabbit
83%
Moderate Homology
Dog
79%
Moderate Homology
Cow
76%
Moderate Homology
Sheep
75%
Moderate Homology
Human
72%
Moderate Homology
Monkey
72%
Moderate Homology
Zebrafish
45%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Trim16 antibody
Immunohistochemistry (IHC) protocol for Trim16 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for Trim16 antibody
Publications
Application:
Species:
Intracellular Mechanical Stress-Mediated Autophagy Cell Death via Nanospikes for Cancer Treatment
Published: 2025-10-13
Journal: Advanced Science
Application: IF
Impact Factor: 14.10
Species: Mouse
PMID: 41082270
Cordyceps cicadae polysaccharides attenuate diabetic nephropathy via the miR-30a-3p/TRIM16 axis
Published: 2023-12-27
Journal: Journal of Diabetes Investigation
Application: IHC,IF
Impact Factor: 3.20
Species: Mouse
PMID: 38149724
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Product Promise
We promise Anti-TRIM16 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A09514-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026