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Product Info Summary
| SKU: | A00326-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-TYR (R501) Antibody
SKU/Catalog Number
A00326-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TYR (R501) Antibody catalog # A00326-2. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TYR (R501) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00326-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 470-520 of Human TYR.
Reactive Species
A00326-2 is reactive to TYR in Human, Mouse, Rat
Calculated molecular weight
60.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00326-2 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of TYR (R501) pAb at 1:500 dilution Lane1:A375 whole cell lysate(40ug) Lane2:Podocyte whole cell lysate(40ug) Lane3:Beas-2B whole cell lysate(40ug) Lane4:PMVEC whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)
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IF image of BS1484 stained A375 cells.The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody TYR (R501) pAb #BS1484(1:100) at 10µg/ml overnight at +4°C. The secondary antibody (Green) was Goat Anti-Mouse IgG (H+L) FITC#BS10950 used at a 1/400 dilution for 2h. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue).
Specific Publications For Anti-TYR (R501) Antibody (A00326-2)
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