DAPP1 (Phospho-Tyr139) Colorimetric Cell-Based ELISA Kit

Dapp1 ELISA kit, phospho-specific, cell-based

The DAPP1 (Phospho-Tyr139) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor DAPP1 (Phospho-Tyr139) protein expression profile in cells. The kit can be used for measuring the relative amounts of DAPP1 (Phospho-Tyr139) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DAPP1 (Phospho-Tyr139).

Product Info Summary

SKU: EKC2248
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA
Sample Types: Cell lines

Product Name

DAPP1 (Phospho-Tyr139) Colorimetric Cell-Based ELISA Kit

See all Dapp1 products

SKU/Catalog Number

EKC2248

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The DAPP1 (Phospho-Tyr139) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor DAPP1 (Phospho-Tyr139) protein expression profile in cells. The kit can be used for measuring the relative amounts of DAPP1 (Phospho-Tyr139) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on DAPP1 (Phospho-Tyr139).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

DAPP1 (Phospho-Tyr139) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2248)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2248 is reactive to Dapp1 in Human, Mouse samples

Validated Sample Types

Cell lines

Application Guarantee

EKC2248 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Dapp1 (Phospho-specific) Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-Dapp1 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Gene/Protein Information For Dapp1 (Source: Uniprot.Org, NCBI)

Gene Name

Dapp1

Full Name

Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide

Weight

32.167kDa

Alternative Names

B lymphocyte adapter protein Bam32; BAM32; BAM32DKFZp667E0716; B-cell adapter molecule of 32 kDa; DAPP1; dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; dual adaptor of phosphotyrosine and 3-phosphoinositides; hDAPP1 Dapp1|Bam3, Bam32|dual adaptor for phosphotyrosine and 3-phosphoinositides 1|dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide|B-cell adapter molecule of 32 kDa|b lymphocyte adapter protein Bam32

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Dapp1, check out the Dapp1 Infographic

Dapp1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Dapp1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EKC2248

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used DAPP1 (Phospho-Tyr139) Colorimetric Cell-Based ELISA Kit?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

Be the first to review DAPP1 (Phospho-Tyr139) Colorimetric Cell-Based ELISA Kit

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for DAPP1 (Phospho-Tyr139) Colorimetric Cell-Based ELISA Kit

$540
Backordered.

Lead time for this item is typically 5-7 days

Get A Quote
In stock
Order Product
EKC2248
$540.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.