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Product Info Summary
| SKU: | P04881 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho RGS16 (Tyr168) Antibody
SKU/Catalog Number
P04881
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho RGS16 (Tyr168) Antibody (Catalog# P04881). Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat, Monkey.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho RGS16 (Tyr168) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P04881)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RGS16 around the phosphorylation site of Tyr168. Immunogen sequence location: 141-190
Reactive Species
P04881 is reactive to RGS16 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
22.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P04881 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 2000-20000
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using RGS16 (Phospho-Tyr168) Antibody
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Western blot analysis of lysates from COS7 cells treated with heat shock, using RGS16 (Phospho-Tyr168) Antibody. The lane on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho RGS16 (Tyr168) Antibody (P04881)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Phospho RGS16 (Tyr168) Antibody
Question
Will P04881 anti-Phospho RGS16 (Tyr168) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
As indicated on the product datasheet, P04881 anti-Phospho RGS16 (Tyr168) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-01
Question
I am interested in to test anti-Phospho RGS16 (Tyr168) antibody P04881 on rat brain for research purposes, then I may be interested in using anti-Phospho RGS16 (Tyr168) antibody P04881 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
The products we sell, including anti-Phospho RGS16 (Tyr168) antibody P04881, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-23
Question
Is there a BSA free version of anti-Phospho RGS16 (Tyr168) antibody P04881 available?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho RGS16 (Tyr168) antibody P04881 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-22
Question
My question regarding product P04881, anti-Phospho RGS16 (Tyr168) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P04881 anti-Phospho RGS16 (Tyr168) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-13
Question
We are currently using anti-Phospho RGS16 (Tyr168) antibody P04881 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
G. Wu
Verified customer
Asked: 2017-03-02
Answer
The anti-Phospho RGS16 (Tyr168) antibody (P04881) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-03-02
