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Product Info Summary
| SKU: | A06385 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Uba5/Ufm1 Activating Enzyme Antibody
SKU/Catalog Number
A06385
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Uba5/Ufm1 Activating Enzyme Antibody catalog # A06385. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Uba5/Ufm1 Activating Enzyme Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06385)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human UBA5. AA range:281-330
Reactive Species
A06385 is reactive to UBA5 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
44.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06385 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Uba5 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysates from HepG2 and 293 cells, using UBA5 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Uba5/Ufm1 Activating Enzyme Antibody (A06385)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Uba5/Ufm1 Activating Enzyme Antibody
Question
Is this A06385 anti-Uba5/Ufm1 Activating Enzyme antibody reactive to the isotypes of UBA5?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
The immunogen of A06385 anti-Uba5/Ufm1 Activating Enzyme antibody is Synthesized peptide derived from the C-terminal region of human Uba5. at AA rangle: 250-330. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-27
Question
See attached the WB image, lot number and protocol we used for lung {eco:0000312 using anti-Uba5/Ufm1 Activating Enzyme antibody A06385. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-03
Question
We want to test anti-Uba5/Ufm1 Activating Enzyme antibody A06385 on human lung {eco:0000312 for research purposes, then I may be interested in using anti-Uba5/Ufm1 Activating Enzyme antibody A06385 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
The products we sell, including anti-Uba5/Ufm1 Activating Enzyme antibody A06385, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-26
Question
We are currently using anti-Uba5/Ufm1 Activating Enzyme antibody A06385 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
The anti-Uba5/Ufm1 Activating Enzyme antibody (A06385) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-29
