Human MICA ELISA Kit PicoKine™

Human MICA ELISA Kit PicoKine™ (96 Tests). Quantitate Human MICA in cell culture supernatants, cell lysates and serum. . Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0812
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates and serum.

Product Name

Human MICA ELISA Kit PicoKine™

See all MICA products

SKU/Catalog Number

EK0812

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MICA ELISA Kit PicoKine™ (96 Tests). Quantitate Human MICA in cell culture supernatants, cell lysates and serum. . Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human MICA ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0812)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E24-W307

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0812 is reactive to MICA in Human samples

Validated Sample Types

cell culture supernatants, cell lysates and serum.

Application Guarantee

EK0812 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0812-CAP Anti-Human MICA Pre-coated 96-well strip microplate 1
EK0812-ST Human MICA Standard 2 vials, 10 ng/tube
EK0812-DA Human MICA Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0450.1140.1650.2870.4700.7921.2281.945

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MICA ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)15157416701655701549
Standard deviation11.1741.32120.2414.0246.74119.27
CV (%)7.4%7.2%7.2%8.5%8.2%7.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MICA in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11511381571441477.154.8%
Sample 257464353454057243.337.5%
Sample 316701895166318611772106.456%
*number of samples for each test n=16.

Gene/Protein Information For MICA (Source: Uniprot.Org, NCBI)

Uniprot ID

Q29983

Gene ID

100507436

Gene Name

MICA

Full Name

MHC class I polypeptide-related sequence A

Weight

42.915kDa

Superfamily

MHC class I family

Alternative Names

FLJ60820; MGC111087; MICA; PERB11.1 MICA MIC-A, PERB11.1 MHC class I polypeptide-related sequence A MHC class I polypeptide-related sequence A|HLA class I antigen|MHC class I chain-related protein A|MHC class I related chain A|MHC class I related sequence A|major histocompatibility complex class I chain-related protein A|stress inducible class I homolog|truncated MHC class I polypeptide-related sequence A|truncated MICA

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MICA, check out the MICA Infographic

MICA infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MICA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Human MICA ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-06-12

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-06-12

Question

Q: how do I analyze ELISA data? I have obtained MICA level in cell lysates.

M. Edwards

Verified customer

Asked: 2018-05-28

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-05-28

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis MICA using the EK0812 Human MICA Picokine® ELISA Kit?

H. Varadkar

Verified customer

Asked: 2018-01-05

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2018-01-05

Question

Q: if the enzyme conjugated MICA antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MICA antigen?

Verified Customer

Verified customer

Asked: 2016-03-06

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-03-06

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