Click image to see more details
Product Info Summary
| SKU: | A01769-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-UCP3 Antibody
SKU/Catalog Number
A01769-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-UCP3 Antibody catalog # A01769-1. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UCP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01769-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human UCP3. AA range:259-308
Reactive Species
A01769-1 is reactive to UCP3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
34.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01769-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using UCP3 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from Jurkat cells, using UCP3 antibody.
Specific Publications For Anti-UCP3 Antibody (A01769-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-UCP3 Antibody
Question
We are currently using anti-UCP3 antibody A01769-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The anti-UCP3 antibody (A01769-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-29
Question
Is a blocking peptide available for product anti-UCP3 antibody (A01769-1)?
P. Parker
Verified customer
Asked: 2018-12-07
Answer
We do provide the blocking peptide for product anti-UCP3 antibody (A01769-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-12-07
Question
Do you have a BSA free version of anti-UCP3 antibody A01769-1 available?
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-UCP3 antibody A01769-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-01
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skeletal muscle using anti-UCP3 antibody A01769-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-18
Question
I have attached the WB image, lot number and protocol we used for skeletal muscle using anti-UCP3 antibody A01769-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-12-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-04
Question
I see that the anti-UCP3 antibody A01769-1 works with WB, what is the protocol used to produce the result images on the product page?
L. Taylor
Verified customer
Asked: 2016-06-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-06-03
Question
Would anti-UCP3 antibody A01769-1 work on bovine WB with gastrocnemius?
L. Bhatt
Verified customer
Asked: 2015-06-15
Answer
Our lab technicians have not validated anti-UCP3 antibody A01769-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-UCP3 antibody A01769-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine gastrocnemius in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-06-15
