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Product Info Summary
| SKU: | M03786-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-USP10 Mouse Monoclonal Antibody [Clone ID: OTI2E1]
SKU/Catalog Number
M03786-1
Size
100 µl
Description
Boster Bio USP10 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1). Catalog# M03786-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP10 Mouse Monoclonal Antibody [Clone ID: OTI2E1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03786-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2E1
Isotype
IgG1
Immunogen
Full length human recombinant protein of human USP10 (NP_005144) produced in HEK293T cell.
Reactive Species
M03786-1 is reactive to USP10 in Dog, Human, Monkey, Mouse, Rat
Observed Molecular Weight
87 kDa
Calculated molecular weight
87.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03786-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500~2000
IHC: 1:150
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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Western blot analysis of USP10 using anti-USP10 antibody (M03786-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-USP10 antigen affinity purified monoclonal antibody (Catalog # M03786-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for USP10 at approximately 110 kDa. The expected band size for USP10 is at 87 kDa.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP10 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP10.
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-USP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Anti-USP10 mouse monoclonal antibody (M03786-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY USP10.
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Flow cytometric Analysis of Hela cells
Specific Publications For Anti-USP10 Mouse Monoclonal Antibody [Clone ID: OTI2E1] (M03786-1)
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