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Product Info Summary
| SKU: | A03786 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-USP10 Antibody
SKU/Catalog Number
A03786
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-USP10 Antibody (Catalog # A03786). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
USP10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-USP10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03786)
Host
Rabbit
Contents
USP10 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
USP10 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human USP10. The immunogen is located within amino acids 30 - 80 of USP10.
Reactive Species
A03786 is reactive to USP10 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
87.1 kDa
Background of USP10
USP10, also known as ubiquitin specific peptidase 10, belongs to the ubiquitin-specific protease family of cysteine proteases. USP10 functions to catalyze the cleavage of ubiquitin from ubiquitin-conjugated protein substrates such as p53/TP53, SNX3 and CFTR. USP10 has been identified as a subunit of DNA-bound androgen receptor (AR) complexes and may play a role in the activity of the DNA-bound androgen receptor complex. USP10 also acts as an essential regulator of p53/TP53 stability and is thought to function as a tumor suppressor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03786 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
USP10 antibody can be used for detection of USP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of USP10 in Jurkat cell lysate with USP10 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL.
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Immunofluorescence of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL.
Specific Publications For Anti-USP10 Antibody (A03786)
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