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- Table of Contents
USP16 antibody (A05795) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Monkey, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of A431 cells using anti-USP16 antibody (A05795).
Overlay histogram showing A431 cells stained with A05795 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-USP16 Antibody (A05795,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with A05795 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-USP16 Antibody (A05795,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-USP16 Antibody Picoband®
USP16 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
USP16 antibody was positively detected by WB in:
HepG2 cell, human Hela whole cell, human U20S whole cell, human T-47D whole cell, human CACO-2 whole cell, monkey heart tissue, monkey kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
USP16 antibody was positively detected by IHC in:
Human brain stem cells, human lung cancer tissue, rat intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
USP16 antibody was positively detected by ICC/IF in:
Hela cell
USP16 antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human, Monkey
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Tested
Recommend
Rat
Tested
Recommend
Recommend
Dog
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-USP16 Antibody Picoband®
View all USP16 Antibodies
View all USP16 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human USP16 recombinant protein (Position: K9-L823).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
100 kDa
Calculated Molecular Weight
93.6 kDa
Background of USP16
Ubiquitin carboxyl-terminal hydrolase 16 is an enzyme that in humans is encoded by the USP16 gene. It is mapped to 21q21.3. This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Cite This Product
Anti-USP16 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05795)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
92%
High Homology
Sheep
92%
High Homology
Cow
91%
High Homology
Rabbit
91%
High Homology
Rat
84%
Moderate Homology
Mouse
83%
Moderate Homology
Zebrafish
50%
Low homology
Drosophila
36%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for USP16 antibody
Immunohistochemistry (IHC) protocol for USP16 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for USP16 antibody
Flow Cytometry (FC) protocol for USP16 antibody
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Product Promise
We promise Anti-USP16 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A05795
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026