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Product Info Summary
| SKU: | M02594 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-USP9X Mouse Monoclonal Antibody [Clone ID: OTI2G7]
SKU/Catalog Number
M02594
Size
100 µl
Description
Boster Bio USP9X mouse monoclonal antibody, clone OTI2G7 (formerly 2G7). Catalog# M02594. Tested in IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-USP9X Mouse Monoclonal Antibody [Clone ID: OTI2G7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02594)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI2G7
Isotype
IgG2a
Immunogen
Human recombinant protein fragment corresponding to amino acids 2246-2570 of human USP9X (NP_001034680) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02594 is reactive to USP9X in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
292.28kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02594 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-USP9X mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-USP9X mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-USP9X mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-USP9X mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-USP9X mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-USP9X mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-USP9X mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-USP9X mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP9X (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP9X.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-USP9X monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and USP9X-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-USP9X monoclonal antibody M02594 (1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Protein Target Info & Infographic
Gene/Protein Information For USP9X (Source: Uniprot.org, NCBI)
Gene Name
USP9X
Full Name
Probable ubiquitin carboxyl-terminal hydrolase FAF-X
Weight
292.28kDa
Superfamily
peptidase C19 family
Alternative Names
Deubiquitinating enzyme FAF-X; DFFRX; DFFRXubiquitin specific protease 9, X-linked (fat facets-like, Drosophila); Drosophila fat facets related, X-linked; EC 3.1.2.15; EC 3.4.19.12; FAF; FAM; Fat facets in mammals; fat facets protein related, X-linked; Fat facets protein-related, X-linked; hFAM; probable ubiquitin carboxyl-terminal hydrolase FAF-X; ubiquitin specific peptidase 9, X-linked (fat facets-like, Drosophila); ubiquitin specific peptidase 9, X-linked; ubiquitin thioesterase FAF-X; Ubiquitin thiolesterase FAF-X; ubiquitin-specific processing protease FAF-X; Ubiquitin-specific protease USP9X DFFRX, FAF, FAM, MRX99, MRXS99F ubiquitin specific peptidase 9 X-linked probable ubiquitin carboxyl-terminal hydrolase FAF-X|Drosophila fat facets related, X-linked|deubiquitinating enzyme FAF-X|fat facets in mammals|fat facets protein related, X-linked|hFAM|ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila)|ubiquitin thioesterase FAF-X|ubiquitin thiolesterase FAF-X|ubiquitin-specific processing protease FAF-X|ubiquitin-specific protease 9, X chromosome
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on USP9X, check out the USP9X Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for USP9X: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-USP9X Mouse Monoclonal Antibody [Clone ID: OTI2G7] (M02594)
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