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Product Info Summary
|Reactive Species:||Mouse, Rat|
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Anti-VEGF/Vegfa Antibody Picoband™
Boster Bio Anti-VEGF/Vegfa Antibody Picoband™ catalog # A00045. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGF/Vegfa Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00045)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived mouse VEGF recombinant protein (Position: A27-R214). Mouse VEGF shares 89.4% and 98.4% amino acid (aa) sequence identity with human and rat VEGF, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00045 is reactive to Vegfa in Mouse, Rat
A00045 is guaranteed for WB Boster Guarantee
Background of Vegfa
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. And VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. This gene can also regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of VEGF using anti-VEGF antibody (A00045).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF antigen affinity purified polyclonal antibody (Catalog # A00045) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF at approximately 27 kDa.
Gene/Protein Information For Vegfa (Source: Uniprot.Org, NCBI)
Vascular endothelial growth factor A
PDGF/VEGF growth factor family
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor Vegfa|V, VEGF12, VEGF16, VEGF18, Veg, Vegf, Vpf|vascular endothelial growth factor A|vascular endothelial growth factor A|vascular permeability factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Vegfa, check out the Vegfa Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Vegfa: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A00045 has been cited in 52 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Id1 induces tubulogenesis by regulating endothelial cell adhesion and cytoskeletal organization through β1-integrin and Rho-kinase signalling
Dexmedetomidine protects against oxygen–glucose deprivation‐induced injury through the I2 imidazoline receptor‐PI3K/AKT pathway in rat C6 glioma cells
Blockage of the formation of new blood vessels by recombinant human endostatin contributes to the regression of rat adjuvant arthritis
Zhou D,Huang X,Xie Y,Deng Z,Guo J,Huang H.Astrocytes-derived VEGF exacerbates the microvascular damage of late delayed RBI.Neuroscience.2019 Jun 1;408:14-21.doi:10.1016/j.neuroscience.2019.03.039.Epub 2019 Mar 22.PMID:30910640.
A00045 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:300
Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration
Inhibition of angiogenesis by arsenic trioxide via TSP-1?TGF-?1-CTGF?VEGF functional module in rheumatoid arthritis
Epidermal growth factor receptor transactivation is involved in the induction of human hepatoma SMMC7721 cell proliferation by insufficient radiofrequency ablation
miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression
IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma
Vitamin K2 Ameliorates Damage of Blood Vessels by Glucocorticoid: a Potential Mechanism for Its Protective Effects in Glucocorticoid-induced Osteonecrosis of the Femoral Head in a Rat Model
Proper autophagy is indispensable for angiogenesis during chick embryo development
VEGFR-2 conformational switch in response to ligand binding
Effect of CPU-XT-008, a combretastatin A-4 analogue, on the proliferation, apoptosis and expression of vascular endothelial growth factor and basic fibroblast growth factor in human umbilical vein endothelial cells
Arginine ADP-ribosyltransferase 1 promotes angiogenesis in colorectal cancer via the PI3K/Akt pathway
Effect of bone mesenchymal stem cells transplantation on the micro-environment of early osteonecrosis of the femoral head
Involvement of MicroRNA-210 Demethylation in Steroid-associated Osteonecrosis of the Femoral Head
The Neuropilin-1 Inhibitor, ATWLPPR Peptide, Prevents Experimental Diabetes-Induced Retinal Injury by Preserving Vascular Integrity and Decreasing Oxidative Stress
Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction
Effects of Feijining Decoction on vascular endothelial growth factor protein expression and changes of T cell subsets in Lewis lung carcinoma-bearing mice
Insufficient radiofrequency ablation promotes human hepatoma SMMC7721 cell proliferation by stimulating vascular endothelial growth factor overexpression
Ectopic expression of AP-2? transcription factor suppresses glioma progression
microRNA-146a inhibits cancer metastasis by downregulating VEGF through dual pathways in hepatocellular carcinoma
Decreased TIP30 Expression Promotes Tumor Metastasis in Lung Cancer
HGF-Transgenic MSCs Can Improve the Effects of Tissue Self-Repair in a Rabbit Model of Traumatic Osteonecrosis of the Femoral Head
Roxarsone induces angiogenesis via PI3K/Akt signaling
Recombinant human endostatin reduces hypertrophic scar formation in rabbit ear model through down-regulation of VEGF and TIMP-1
Matrix metalloproteinase-9 and vascular endothelial growth factor expression change in experimental retinal neovascularization
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
Buyanghuanwu decoction promotes angiogenesis after cerebral ischemia/reperfusion injury: mechanisms of brain tissue repair
Lentivirus-mediated PLC?1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma
Correlation and Significance of Urinary Soluble Fas and Vascular Endothelial Growth Factor in Bladder Urothelial Cancer
Factors influencing improvement of visual field after trans-sphenoidal resection of pituitary macroadenomas: a retrospective cohort study
Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury
Downregulation of VEGF mRNA expression by triamcinolone acetonide acetate-loaded chitosan derivative nanoparticles in human retinal pigment epithelial cells
Qin JH, Ke ZY, Zhou Q, Wang L, Liang Y, Wang YM, Yang T, Gao X, Ye J, Kumar R, Wang RA,. Med Sci Monit. 2017 Aug 15;23:3932-3941. Metastasis-Associated Protein 1 Deficiency Results in Compromised Pulmonary Alveolar Capillary Angiogenesis in Mice
Liu Mw, Su Mx, Zhang W, Wang Yh, Qin Lf, Liu X, Tian Ml, Qian Cy. Mol Med Rep. 2015 May;11(5):3308-16. Doi: 10.3892/Mmr.2015.3146. Epub 2015 Jan 7. Effect Of Melilotus Suaveolens Extract On Pulmonary Microvascular Permeability By Downregulating Va...
Jin M, Zhang Y, Pan L, Dou R, Nussenblatt Rb, Wei L. J Transl Med. 2012 Jun 7;10:118. Doi: 10.1186/1479-5876-10-118. The Chinese Medicine Formula Hb01 Reduces Choroidal Neovascularization By Regulating The Expression Of Vascular Endothelial Growth...
Li X, Wang H, Lu X, Di B. Exp Ther Med. 2010 Nov;1(6):947-953. Epub 2010 Sep 29. Silencing Stat3 With Short Hairpin Rna Enhances Radiosensitivity Of Human Laryngeal Squamous Cell Carcinoma Xenografts In Vivo.
Hou C, Kong J, Liang Y, Huang H, Wen H, Zheng X, Wu L, Chen Y. Cell Mol Immunol. 2015 Jul;12(4):409-23. Doi: 10.1038/Cmi.2014.60. Epub 2014 Aug 25. Hmgb1 Contributes To Allergen-Induced Airway Remodeling In A Murine Model Of Chronic Asthma By Modu...
Hao Jh, Yu M, Li Hk, Shi Yr, Li Q, Hao Xs. World J Gastroenterol. 2006 Feb 21;12(7):1140-3. Inhibitory Effect Of Antisense Vascular Endothelial Growth Factor Rna On The Profile Of Hepatocellular Carcinoma Cell Line In Vitro And In Vivo.
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...
Kong J, Kong J, Pan B, Ke S, Dong S, Li X, Zhou A, Zheng L, Sun Wb. Plos One. 2012;7(5):E37266. Doi: 10.1371/Journal.Pone.0037266. Epub 2012 May 15. Insufficient Radiofrequency Ablation Promotes Angiogenesis Of Residual Hepatocellular Carcinoma Vi...
Li Jk, Yu L, Shen Y, Zhou Ls, Wang Yc, Zhang Jh. World J Gastroenterol. 2008 Apr 21;14(15):2308-13. Inhibition Of Cxcr4 Activity With Amd3100 Decreases Invasion Of Human Colorectal Cancer Cells In Vitro.
Ran J, Ma J, Liu Y, Tan R, Liu H, Lao G. J Diabetes Res. 2014;2014:287536. Doi: 10.1155/2014/287536. Epub 2014 Mar 13. Low Protein Diet Inhibits Uric Acid Synthesis And Attenuates Renal Damage In Streptozotocin-Induced Diabetic Rats.
Hu J, Zhou Z, Shi S, Zhu X, Wang X, Zhang W, Hu S, Qian H, Xu W. Oncol Lett. 2013 Jan;5(1):179-184. Epub 2012 Oct 30. Mesenchymal Stem-Like Cells Isolated From Human Esophageal Carcinoma And Adjacent Non-Cancerous Tissues.
Duan F, Lin M, Li C, Ding X, Qian G, Zhang H, Ge S, Fan X, Li J. Cancer Biol Ther. 2014 May;15(5):544-59. Doi: 10.4161/Cbt.28157. Epub 2014 Mar 11. Effects Of Inhibition Of Hedgehog Signaling On Cell Growth And Migration Of Uveal Melanoma Cells.
Lin J, Li G, Den X, Xu C, Liu S, Gao Y, Liu H, Zhang J, Li X, Liang S. Brain Res Bull. 2010 Oct 30;83(5):284-91. Doi: 10.1016/J.Brainresbull.2010.08.002. Epub 2010 Aug 10. Vegf And Its Receptor-2 Involved In Neuropathic Pain Transmission Mediated ...
Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1
Song D, Yue W, Li Z, Li J, Zhao J, Zhang N. Onco Targets Ther. 2014 Sep 30;7:1801-10. Doi: 10.2147/Ott.S52426. Ecollection 2014. Study Of The Mechanism Of Sonodynamic Therapy In A Rat Glioma Model.
Ge Y, Ding Y, Zhang J, Li Z, Li Z. Int J Clin Exp Med. 2014 Oct 15;7(10):3578-82. Ecollection 2014. Effect Of Angiogenesis Inhibitor Su6668 In Combination With 5-Fu On Liver Metastasis From Transplantation Tumors Of Human Colorectal Cancer In Nude...
Tong J, Ding J, Shen X, Chen L, Bian Y, Ma G, Yao Y, Yang F. Plos One. 2013 Nov 14;8(11):E80186. Doi: 10.1371/Journal.Pone.0080186. Ecollection 2013. Mesenchymal Stem Cell Transplantation Enhancement In Myocardial Infarction Rat Model Under Ultras...
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