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Product Info Summary
|Reactive Species:||Mouse, Rat|
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Anti-VEGF/Vegfa Antibody Picoband™
Boster Bio Anti-VEGF/Vegfa Antibody Picoband™ catalog # A00045. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGF/Vegfa Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00045)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived mouse VEGF recombinant protein (Position: A27-R214). Mouse VEGF shares 89.4% and 98.4% amino acid (aa) sequence identity with human and rat VEGF, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00045 is reactive to Vegfa in Mouse, Rat
A00045 is guaranteed for WB Boster Guarantee
Background of Vegfa
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. And VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. This gene can also regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of VEGF using anti-VEGF antibody (A00045).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF antigen affinity purified polyclonal antibody (Catalog # A00045) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF at approximately 27 kDa.
Gene/Protein Information For Vegfa (Source: Uniprot.org, NCBI)
Vascular endothelial growth factor A
PDGF/VEGF growth factor family
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor Vegfa|V, VEGF12, VEGF16, VEGF18, Veg, Vegf, Vpf|vascular endothelial growth factor A|vascular endothelial growth factor A|vascular permeability factor*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Vegfa, check out the Vegfa Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Vegfa: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
A00045 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Id1 induces tubulogenesis by regulating endothelial cell adhesion and cytoskeletal organization through β1-integrin and Rho-kinase signalling
Dexmedetomidine protects against oxygen–glucose deprivation‐induced injury through the I2 imidazoline receptor‐PI3K/AKT pathway in rat C6 glioma cells
Blockage of the formation of new blood vessels by recombinant human endostatin contributes to the regression of rat adjuvant arthritis
Zhou D,Huang X,Xie Y,Deng Z,Guo J,Huang H.Astrocytes-derived VEGF exacerbates the microvascular damage of late delayed RBI.Neuroscience.2019 Jun 1;408:14-21.doi:10.1016/j.neuroscience.2019.03.039.Epub 2019 Mar 22.PMID:30910640.
A00045 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:300
Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration
Inhibition of angiogenesis by arsenic trioxide via TSP-1?TGF-?1-CTGF?VEGF functional module in rheumatoid arthritis
Epidermal growth factor receptor transactivation is involved in the induction of human hepatoma SMMC7721 cell proliferation by insufficient radiofrequency ablation
miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression
IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma
Vitamin K2 Ameliorates Damage of Blood Vessels by Glucocorticoid: a Potential Mechanism for Its Protective Effects in Glucocorticoid-induced Osteonecrosis of the Femoral Head in a Rat Model
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