Anti-VEGF/Vegfa Antibody Picoband™

Vegfa antibody

Boster Bio Anti-VEGF/Vegfa Antibody Picoband™ catalog # A00045. Tested in WB applications. This antibody reacts with Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: A00045
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-VEGF/Vegfa Antibody Picoband™

View all Vegfa Antibodies

SKU/Catalog Number

A00045

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-VEGF/Vegfa Antibody Picoband™ catalog # A00045. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VEGF/Vegfa Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00045)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse VEGF recombinant protein (Position: A27-R214). Mouse VEGF shares 89.4% and 98.4% amino acid (aa) sequence identity with human and rat VEGF, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00045 is reactive to Vegfa in Mouse, Rat

Applications

A00045 is guaranteed for WB Boster Guarantee

Background of Vegfa

VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. And VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. This gene can also regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Vegfa (Source: Uniprot.org, NCBI)

Uniprot ID

Q00731

Gene ID

22339

Gene Name

Vegfa

Full Name

Vascular endothelial growth factor A

Weight

25.283kDa

Superfamily

PDGF/VEGF growth factor family

Alternative Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor Vegfa|V, VEGF12, VEGF16, VEGF18, Veg, Vegf, Vpf|vascular endothelial growth factor A|vascular endothelial growth factor A|vascular permeability factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Vegfa, check out the Vegfa Infographic

Vegfa infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Vegfa: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

A00045 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Id1 induces tubulogenesis by regulating endothelial cell adhesion and cytoskeletal organization through β1-integrin and Rho-kinase signalling

Dexmedetomidine protects against oxygen–glucose deprivation‐induced injury through the I2 imidazoline receptor‐PI3K/AKT pathway in rat C6 glioma cells

Blockage of the formation of new blood vessels by recombinant human endostatin contributes to the regression of rat adjuvant arthritis

Zhou D,Huang X,Xie Y,Deng Z,Guo J,Huang H.Astrocytes-derived VEGF exacerbates the microvascular damage of late delayed RBI.Neuroscience.2019 Jun 1;408:14-21.doi:10.1016/j.neuroscience.2019.03.039.Epub 2019 Mar 22.PMID:30910640.
Species: Mouse
A00045 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:300

Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration

Inhibition of angiogenesis by arsenic trioxide via TSP-1?TGF-?1-CTGF?VEGF functional module in rheumatoid arthritis

Epidermal growth factor receptor transactivation is involved in the induction of human hepatoma SMMC7721 cell proliferation by insufficient radiofrequency ablation

miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression

IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma

Vitamin K2 Ameliorates Damage of Blood Vessels by Glucocorticoid: a Potential Mechanism for Its Protective Effects in Glucocorticoid-induced Osteonecrosis of the Femoral Head in a Rat Model

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15 Customer Q&As for Anti-VEGF/Vegfa Antibody Picoband™

Question

Would A00045 anti-VEGF/Vegfa antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-05-05

Answer

As indicated on the product datasheet, A00045 anti-VEGF/Vegfa antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-05-05

Question

I am interested in to test anti-VEGF/Vegfa antibody A00045 on rat mammary gland for research purposes, then I may be interested in using anti-VEGF/Vegfa antibody A00045 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

The products we sell, including anti-VEGF/Vegfa antibody A00045, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-03

Question

Does anti-VEGF/Vegfa antibody A00045 work for WB with mammary gland?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

According to the expression profile of mammary gland, VEGFA is highly expressed in mammary gland. So, it is likely that anti-VEGF/Vegfa antibody A00045 will work for WB with mammary gland.

Boster Scientific Support

Answered: 2019-10-10

Question

I am interested in using your anti-VEGF/Vegfa antibody for negative regulation of neuron death studies. Has this antibody been tested with western blotting on rat kidney? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-09-16

Answer

We appreciate your inquiry. This A00045 anti-VEGF/Vegfa antibody is tested on rat kidney. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-09-16

Question

My question regarding product A00045, anti-VEGF/Vegfa antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00045 anti-VEGF/Vegfa antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-05

Question

Do you have a BSA free version of anti-VEGF/Vegfa antibody A00045 available?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VEGF/Vegfa antibody A00045 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-16

Question

Is this A00045 anti-VEGF/Vegfa antibody reactive to the isotypes of VEGFA?

Verified Customer

Verified customer

Asked: 2018-12-10

Answer

The immunogen of A00045 anti-VEGF/Vegfa antibody is E.coli-derived mouse VEGF recombinant protein (Position: A27-R214). Mouse VEGF shares 89.4% and 98.4% amino acid (aa) sequence identity with human and rat VEGF, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-12-10

Question

We are currently using anti-VEGF/Vegfa antibody A00045 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2018-10-19

Answer

The anti-VEGF/Vegfa antibody (A00045) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-19

Question

We were happy with the WB result of your anti-VEGF/Vegfa antibody. However we have been able to see positive staining in lung secreted using this antibody. Is that expected? Could you tell me where is VEGFA supposed to be expressed?

C. Moore

Verified customer

Asked: 2018-10-11

Answer

According to literature, lung does express VEGFA. Generally VEGFA expresses in secreted. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986

Boster Scientific Support

Answered: 2018-10-11

Question

See below the WB image, lot number and protocol we used for mammary gland using anti-VEGF/Vegfa antibody A00045. Please let me know if you require anything else.

J. Krishna

Verified customer

Asked: 2018-08-22

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-08-22

Question

We have observed staining in rat renal glomerulus. Do you have any suggestions? Is anti-VEGF/Vegfa antibody supposed to stain renal glomerulus positively?

K. Jackson

Verified customer

Asked: 2018-06-25

Answer

Based on literature renal glomerulus does express VEGFA. Based on Uniprot.org, VEGFA is expressed in left lobe of thyroid gland, kidney, mammary gland, renal glomerulus, hemangioendothelioma, heart, lung, retina, among other tissues. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986

Boster Scientific Support

Answered: 2018-06-25

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary gland using anti-VEGF/Vegfa antibody A00045. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-03-06

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-03-06

Question

Is a blocking peptide available for product anti-VEGF/Vegfa antibody (A00045)?

Verified Customer

Verified customer

Asked: 2017-08-04

Answer

We do provide the blocking peptide for product anti-VEGF/Vegfa antibody (A00045). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-08-04

Question

I see that the anti-VEGF/Vegfa antibody A00045 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-06-16

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-06-16

Question

I was wanting to use your anti-VEGF/Vegfa antibody for WB for rat mammary gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat mammary gland identification?

R. Singh

Verified customer

Asked: 2016-05-24

Answer

You can see on the product datasheet, A00045 anti-VEGF/Vegfa antibody has been validated for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat mammary gland in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-05-24

Size

Conjugation

Total: $315

SKU:A00045

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A00045
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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