Click image to see more details
Product Info Summary
| SKU: | A00045 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-VEGF/Vegfa Antibody Picoband™
SKU/Catalog Number
A00045
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VEGF/Vegfa Antibody Picoband™ catalog # A00045. Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGF/Vegfa Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00045)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Immunogen
E.coli-derived mouse VEGF recombinant protein (Position: A27-R214). Mouse VEGF shares 89.4% and 98.4% amino acid (aa) sequence identity with human and rat VEGF, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross Reactivity
No cross reactivity with other proteins.
Reactive Species
A00045 is reactive to Vegfa in Mouse, Rat
Applications
A00045 is guaranteed for WB Boster Guarantee
Background of Vegfa
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. And VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. This gene can also regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of VEGF using anti-VEGF antibody (A00045).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF antigen affinity purified polyclonal antibody (Catalog # A00045) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF at approximately 27 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Vegfa (Source: Uniprot.Org, NCBI)
Uniprot ID
Q00731
Gene ID
22339
Gene Name
Vegfa
Full Name
Vascular endothelial growth factor A
Weight
25.283kDa
Superfamily
PDGF/VEGF growth factor family
Alternative Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor Vegfa|V, VEGF12, VEGF16, VEGF18, Veg, Vegf, Vpf|vascular endothelial growth factor A|vascular endothelial growth factor A|vascular permeability factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Vegfa, check out the Vegfa Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Vegfa: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-VEGF/Vegfa Antibody Picoband™ (A00045)
A00045 has been cited in 52 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Id1 induces tubulogenesis by regulating endothelial cell adhesion and cytoskeletal organization through β1-integrin and Rho-kinase signalling
Dexmedetomidine protects against oxygen–glucose deprivation‐induced injury through the I2 imidazoline receptor‐PI3K/AKT pathway in rat C6 glioma cells
Blockage of the formation of new blood vessels by recombinant human endostatin contributes to the regression of rat adjuvant arthritis
Zhou D,Huang X,Xie Y,Deng Z,Guo J,Huang H.Astrocytes-derived VEGF exacerbates the microvascular damage of late delayed RBI.Neuroscience.2019 Jun 1;408:14-21.doi:10.1016/j.neuroscience.2019.03.039.Epub 2019 Mar 22.PMID:30910640.
Species: Mouse
A00045 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:300
Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration
Inhibition of angiogenesis by arsenic trioxide via TSP-1?TGF-?1-CTGF?VEGF functional module in rheumatoid arthritis
Epidermal growth factor receptor transactivation is involved in the induction of human hepatoma SMMC7721 cell proliferation by insufficient radiofrequency ablation
miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression
IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma
Vitamin K2 Ameliorates Damage of Blood Vessels by Glucocorticoid: a Potential Mechanism for Its Protective Effects in Glucocorticoid-induced Osteonecrosis of the Femoral Head in a Rat Model
Proper autophagy is indispensable for angiogenesis during chick embryo development
VEGFR-2 conformational switch in response to ligand binding
Effect of CPU-XT-008, a combretastatin A-4 analogue, on the proliferation, apoptosis and expression of vascular endothelial growth factor and basic fibroblast growth factor in human umbilical vein endothelial cells
Arginine ADP-ribosyltransferase 1 promotes angiogenesis in colorectal cancer via the PI3K/Akt pathway
Effect of bone mesenchymal stem cells transplantation on the micro-environment of early osteonecrosis of the femoral head
Involvement of MicroRNA-210 Demethylation in Steroid-associated Osteonecrosis of the Femoral Head
The Neuropilin-1 Inhibitor, ATWLPPR Peptide, Prevents Experimental Diabetes-Induced Retinal Injury by Preserving Vascular Integrity and Decreasing Oxidative Stress
Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction
Effects of Feijining Decoction on vascular endothelial growth factor protein expression and changes of T cell subsets in Lewis lung carcinoma-bearing mice
Insufficient radiofrequency ablation promotes human hepatoma SMMC7721 cell proliferation by stimulating vascular endothelial growth factor overexpression
Ectopic expression of AP-2? transcription factor suppresses glioma progression
microRNA-146a inhibits cancer metastasis by downregulating VEGF through dual pathways in hepatocellular carcinoma
Decreased TIP30 Expression Promotes Tumor Metastasis in Lung Cancer
HGF-Transgenic MSCs Can Improve the Effects of Tissue Self-Repair in a Rabbit Model of Traumatic Osteonecrosis of the Femoral Head
Roxarsone induces angiogenesis via PI3K/Akt signaling
Recombinant human endostatin reduces hypertrophic scar formation in rabbit ear model through down-regulation of VEGF and TIMP-1
Matrix metalloproteinase-9 and vascular endothelial growth factor expression change in experimental retinal neovascularization
A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
Buyanghuanwu decoction promotes angiogenesis after cerebral ischemia/reperfusion injury: mechanisms of brain tissue repair
Lentivirus-mediated PLC?1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma
Correlation and Significance of Urinary Soluble Fas and Vascular Endothelial Growth Factor in Bladder Urothelial Cancer
Factors influencing improvement of visual field after trans-sphenoidal resection of pituitary macroadenomas: a retrospective cohort study
Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury
Downregulation of VEGF mRNA expression by triamcinolone acetonide acetate-loaded chitosan derivative nanoparticles in human retinal pigment epithelial cells
Qin JH, Ke ZY, Zhou Q, Wang L, Liang Y, Wang YM, Yang T, Gao X, Ye J, Kumar R, Wang RA,. Med Sci Monit. 2017 Aug 15;23:3932-3941. Metastasis-Associated Protein 1 Deficiency Results in Compromised Pulmonary Alveolar Capillary Angiogenesis in Mice
Liu Mw, Su Mx, Zhang W, Wang Yh, Qin Lf, Liu X, Tian Ml, Qian Cy. Mol Med Rep. 2015 May;11(5):3308-16. Doi: 10.3892/Mmr.2015.3146. Epub 2015 Jan 7. Effect Of Melilotus Suaveolens Extract On Pulmonary Microvascular Permeability By Downregulating Va...
Jin M, Zhang Y, Pan L, Dou R, Nussenblatt Rb, Wei L. J Transl Med. 2012 Jun 7;10:118. Doi: 10.1186/1479-5876-10-118. The Chinese Medicine Formula Hb01 Reduces Choroidal Neovascularization By Regulating The Expression Of Vascular Endothelial Growth...
Li X, Wang H, Lu X, Di B. Exp Ther Med. 2010 Nov;1(6):947-953. Epub 2010 Sep 29. Silencing Stat3 With Short Hairpin Rna Enhances Radiosensitivity Of Human Laryngeal Squamous Cell Carcinoma Xenografts In Vivo.
Hou C, Kong J, Liang Y, Huang H, Wen H, Zheng X, Wu L, Chen Y. Cell Mol Immunol. 2015 Jul;12(4):409-23. Doi: 10.1038/Cmi.2014.60. Epub 2014 Aug 25. Hmgb1 Contributes To Allergen-Induced Airway Remodeling In A Murine Model Of Chronic Asthma By Modu...
Hao Jh, Yu M, Li Hk, Shi Yr, Li Q, Hao Xs. World J Gastroenterol. 2006 Feb 21;12(7):1140-3. Inhibitory Effect Of Antisense Vascular Endothelial Growth Factor Rna On The Profile Of Hepatocellular Carcinoma Cell Line In Vitro And In Vivo.
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
Cai N, Liu Nn, Zhao N, Wan C, Hu Yd, Zhou Y, Chen L. Int J Ophthalmol. 2012;5(3):293-6. Doi: 10.3980/J.Issn.2222-3959.2012.03.08. Epub 2012 Jun 18. Expressions Of Survivin And Vascular Endothelial Growth Factor In A Murine Model Of Proliferative R...
Kong J, Kong J, Pan B, Ke S, Dong S, Li X, Zhou A, Zheng L, Sun Wb. Plos One. 2012;7(5):E37266. Doi: 10.1371/Journal.Pone.0037266. Epub 2012 May 15. Insufficient Radiofrequency Ablation Promotes Angiogenesis Of Residual Hepatocellular Carcinoma Vi...
Li Jk, Yu L, Shen Y, Zhou Ls, Wang Yc, Zhang Jh. World J Gastroenterol. 2008 Apr 21;14(15):2308-13. Inhibition Of Cxcr4 Activity With Amd3100 Decreases Invasion Of Human Colorectal Cancer Cells In Vitro.
Ran J, Ma J, Liu Y, Tan R, Liu H, Lao G. J Diabetes Res. 2014;2014:287536. Doi: 10.1155/2014/287536. Epub 2014 Mar 13. Low Protein Diet Inhibits Uric Acid Synthesis And Attenuates Renal Damage In Streptozotocin-Induced Diabetic Rats.
Hu J, Zhou Z, Shi S, Zhu X, Wang X, Zhang W, Hu S, Qian H, Xu W. Oncol Lett. 2013 Jan;5(1):179-184. Epub 2012 Oct 30. Mesenchymal Stem-Like Cells Isolated From Human Esophageal Carcinoma And Adjacent Non-Cancerous Tissues.
Duan F, Lin M, Li C, Ding X, Qian G, Zhang H, Ge S, Fan X, Li J. Cancer Biol Ther. 2014 May;15(5):544-59. Doi: 10.4161/Cbt.28157. Epub 2014 Mar 11. Effects Of Inhibition Of Hedgehog Signaling On Cell Growth And Migration Of Uveal Melanoma Cells.
Lin J, Li G, Den X, Xu C, Liu S, Gao Y, Liu H, Zhang J, Li X, Liang S. Brain Res Bull. 2010 Oct 30;83(5):284-91. Doi: 10.1016/J.Brainresbull.2010.08.002. Epub 2010 Aug 10. Vegf And Its Receptor-2 Involved In Neuropathic Pain Transmission Mediated ...
Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1
Song D, Yue W, Li Z, Li J, Zhao J, Zhang N. Onco Targets Ther. 2014 Sep 30;7:1801-10. Doi: 10.2147/Ott.S52426. Ecollection 2014. Study Of The Mechanism Of Sonodynamic Therapy In A Rat Glioma Model.
Ge Y, Ding Y, Zhang J, Li Z, Li Z. Int J Clin Exp Med. 2014 Oct 15;7(10):3578-82. Ecollection 2014. Effect Of Angiogenesis Inhibitor Su6668 In Combination With 5-Fu On Liver Metastasis From Transplantation Tumors Of Human Colorectal Cancer In Nude...
Tong J, Ding J, Shen X, Chen L, Bian Y, Ma G, Yao Y, Yang F. Plos One. 2013 Nov 14;8(11):E80186. Doi: 10.1371/Journal.Pone.0080186. Ecollection 2013. Mesenchymal Stem Cell Transplantation Enhancement In Myocardial Infarction Rat Model Under Ultras...
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Customer Q&As
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15 Customer Q&As for Anti-VEGF/Vegfa Antibody Picoband™
Question
Would A00045 anti-VEGF/Vegfa antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
As indicated on the product datasheet, A00045 anti-VEGF/Vegfa antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-05
Question
I am interested in to test anti-VEGF/Vegfa antibody A00045 on rat mammary gland for research purposes, then I may be interested in using anti-VEGF/Vegfa antibody A00045 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The products we sell, including anti-VEGF/Vegfa antibody A00045, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-03
Question
Does anti-VEGF/Vegfa antibody A00045 work for WB with mammary gland?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
According to the expression profile of mammary gland, VEGFA is highly expressed in mammary gland. So, it is likely that anti-VEGF/Vegfa antibody A00045 will work for WB with mammary gland.
Boster Scientific Support
Answered: 2019-10-10
Question
I am interested in using your anti-VEGF/Vegfa antibody for negative regulation of neuron death studies. Has this antibody been tested with western blotting on rat kidney? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
We appreciate your inquiry. This A00045 anti-VEGF/Vegfa antibody is tested on rat kidney. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-16
Question
My question regarding product A00045, anti-VEGF/Vegfa antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00045 anti-VEGF/Vegfa antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-05
Question
Do you have a BSA free version of anti-VEGF/Vegfa antibody A00045 available?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VEGF/Vegfa antibody A00045 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-16
Question
Is this A00045 anti-VEGF/Vegfa antibody reactive to the isotypes of VEGFA?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
The immunogen of A00045 anti-VEGF/Vegfa antibody is E.coli-derived mouse VEGF recombinant protein (Position: A27-R214). Mouse VEGF shares 89.4% and 98.4% amino acid (aa) sequence identity with human and rat VEGF, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-10
Question
We are currently using anti-VEGF/Vegfa antibody A00045 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
The anti-VEGF/Vegfa antibody (A00045) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-19
Question
We were happy with the WB result of your anti-VEGF/Vegfa antibody. However we have been able to see positive staining in lung secreted using this antibody. Is that expected? Could you tell me where is VEGFA supposed to be expressed?
C. Moore
Verified customer
Asked: 2018-10-11
Answer
According to literature, lung does express VEGFA. Generally VEGFA expresses in secreted. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986
Boster Scientific Support
Answered: 2018-10-11
Question
See below the WB image, lot number and protocol we used for mammary gland using anti-VEGF/Vegfa antibody A00045. Please let me know if you require anything else.
J. Krishna
Verified customer
Asked: 2018-08-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-22
Question
We have observed staining in rat renal glomerulus. Do you have any suggestions? Is anti-VEGF/Vegfa antibody supposed to stain renal glomerulus positively?
K. Jackson
Verified customer
Asked: 2018-06-25
Answer
Based on literature renal glomerulus does express VEGFA. Based on Uniprot.org, VEGFA is expressed in left lobe of thyroid gland, kidney, mammary gland, renal glomerulus, hemangioendothelioma, heart, lung, retina, among other tissues. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986
Boster Scientific Support
Answered: 2018-06-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary gland using anti-VEGF/Vegfa antibody A00045. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-06
Question
Is a blocking peptide available for product anti-VEGF/Vegfa antibody (A00045)?
Verified Customer
Verified customer
Asked: 2017-08-04
Answer
We do provide the blocking peptide for product anti-VEGF/Vegfa antibody (A00045). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-04
Question
I see that the anti-VEGF/Vegfa antibody A00045 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-16
Question
I was wanting to use your anti-VEGF/Vegfa antibody for WB for rat mammary gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat mammary gland identification?
R. Singh
Verified customer
Asked: 2016-05-24
Answer
You can see on the product datasheet, A00045 anti-VEGF/Vegfa antibody has been validated for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-24
