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Product Info Summary
| SKU: | A00045 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-VEGF/Vegfa Antibody Picoband®
SKU/Catalog Number
A00045
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-VEGF/Vegfa Antibody Picoband® catalog # A00045. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VEGF/Vegfa Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00045)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse VEGF recombinant protein (Position: A27-R214). Mouse VEGF shares 89.4% and 98.4% amino acid (aa) sequence identity with human and rat VEGF, respectively.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00045 is reactive to Vegfa in Mouse, Rat
Observed Molecular Weight
27 kDa.
Calculated molecular weight
25.3 kDa
Background of Vegfa
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. And VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. This gene can also regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00045 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Mouse
Positive Control
WB: rat kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of VEGF using anti-VEGF antibody (A00045).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF antigen affinity purified polyclonal antibody (Catalog # A00045) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF at approximately 27 kDa.
Specific Publications For Anti-VEGF/Vegfa Antibody Picoband® (A00045)
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Customer Q&As
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15 Customer Q&As for Anti-VEGF/Vegfa Antibody Picoband®
Question
Would A00045 anti-VEGF/Vegfa antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
As indicated on the product datasheet, A00045 anti-VEGF/Vegfa antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-05
Question
I am interested in to test anti-VEGF/Vegfa antibody A00045 on rat mammary gland for research purposes, then I may be interested in using anti-VEGF/Vegfa antibody A00045 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
The products we sell, including anti-VEGF/Vegfa antibody A00045, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-03
Question
Does anti-VEGF/Vegfa antibody A00045 work for WB with mammary gland?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
According to the expression profile of mammary gland, VEGFA is highly expressed in mammary gland. So, it is likely that anti-VEGF/Vegfa antibody A00045 will work for WB with mammary gland.
Boster Scientific Support
Answered: 2019-10-10
Question
I am interested in using your anti-VEGF/Vegfa antibody for negative regulation of neuron death studies. Has this antibody been tested with western blotting on rat kidney? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
We appreciate your inquiry. This A00045 anti-VEGF/Vegfa antibody is tested on rat kidney. It is guaranteed to work for WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-16
Question
My question regarding product A00045, anti-VEGF/Vegfa antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00045 anti-VEGF/Vegfa antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-05
Question
Do you have a BSA free version of anti-VEGF/Vegfa antibody A00045 available?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-VEGF/Vegfa antibody A00045 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-16
Question
Is this A00045 anti-VEGF/Vegfa antibody reactive to the isotypes of VEGFA?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
The immunogen of A00045 anti-VEGF/Vegfa antibody is E.coli-derived mouse VEGF recombinant protein (Position: A27-R214). Mouse VEGF shares 89.4% and 98.4% amino acid (aa) sequence identity with human and rat VEGF, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-10
Question
We are currently using anti-VEGF/Vegfa antibody A00045 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
The anti-VEGF/Vegfa antibody (A00045) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-19
Question
We were happy with the WB result of your anti-VEGF/Vegfa antibody. However we have been able to see positive staining in lung secreted using this antibody. Is that expected? Could you tell me where is VEGFA supposed to be expressed?
C. Moore
Verified customer
Asked: 2018-10-11
Answer
According to literature, lung does express VEGFA. Generally VEGFA expresses in secreted. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986
Boster Scientific Support
Answered: 2018-10-11
Question
See below the WB image, lot number and protocol we used for mammary gland using anti-VEGF/Vegfa antibody A00045. Please let me know if you require anything else.
J. Krishna
Verified customer
Asked: 2018-08-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-22
Question
We have observed staining in rat renal glomerulus. Do you have any suggestions? Is anti-VEGF/Vegfa antibody supposed to stain renal glomerulus positively?
K. Jackson
Verified customer
Asked: 2018-06-25
Answer
Based on literature renal glomerulus does express VEGFA. Based on Uniprot.org, VEGFA is expressed in left lobe of thyroid gland, kidney, mammary gland, renal glomerulus, hemangioendothelioma, heart, lung, retina, among other tissues. Regarding which tissues have VEGFA expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 19054851
Kidney, Pubmed ID: 9878851, 12124351
Lung, Pubmed ID: 15489334
Mammary gland, Pubmed ID: 9450968
Renal glomerulus, Pubmed ID: 10464055
Retina, Pubmed ID: 10067980, 11563986
Boster Scientific Support
Answered: 2018-06-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary gland using anti-VEGF/Vegfa antibody A00045. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-06
Question
Is a blocking peptide available for product anti-VEGF/Vegfa antibody (A00045)?
Verified Customer
Verified customer
Asked: 2017-08-04
Answer
We do provide the blocking peptide for product anti-VEGF/Vegfa antibody (A00045). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-08-04
Question
I see that the anti-VEGF/Vegfa antibody A00045 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-06-16
Question
I was wanting to use your anti-VEGF/Vegfa antibody for WB for rat mammary gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat mammary gland identification?
R. Singh
Verified customer
Asked: 2016-05-24
Answer
You can see on the product datasheet, A00045 anti-VEGF/Vegfa antibody has been validated for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-24
