Human LYVE-1 ELISA Kit PicoKine™

Human LYVE-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human LYVE1 in cell culture supernatants and cell lysates. Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0967
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants and cell lysates.

Product Name

Human LYVE-1 ELISA Kit PicoKine™

See all LYVE-1 products

SKU/Catalog Number

EK0967

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human LYVE-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human LYVE1 in cell culture supernatants and cell lysates. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human LYVE-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0967)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S24-T238

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0967 is reactive to LYVE1 in Human samples

Validated Sample Types

cell culture supernatants and cell lysates.

Application Guarantee

EK0967 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0967-CAP Anti-Human LYVE1 Pre-coated 96-well strip microplate 1
EK0967-ST Human LYVE1 Standard 2 vials, 10 ng/tube
EK0967-DA Human LYVE1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)02000200020002000200020002000
O.D.0.0020.1360.2360.4490.7941.3571.9972.479

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect LYVE-1, Dilution ratio of 1:200, concentration in serum is around 50ng/ml.

Some articles we found to cite concentrations of LYVE-1 in samples: 24665348 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human LYVE-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)8228798778288893
Standard deviation4.5919.2252.315.8519.8763.4
CV (%)6.7%%6.7%5.3%7.5%6.9%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of LYVE-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 182868171805.526.9%
Sample 228726526528427510.33.7%
Sample 398782998899194869.157.2%
*number of samples for each test n=16.

Gene/Protein Information For LYVE1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9Y5Y7

Gene ID

10894

Gene Name

LYVE1

Full Name

Lymphatic vessel endothelial hyaluronic acid receptor 1

Weight

35.213kDa

Alternative Names

cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; LYVE-1XLKD1; XLKD1 LYVE1 CRSBP-1, HAR, LYVE-1, XLKD1 lymphatic vessel endothelial hyaluronan receptor 1 lymphatic vessel endothelial hyaluronic acid receptor 1|cell surface retention sequence binding protein-1|extracellular link domain-containing 1|extracellular link domain-containing protein 1|hyaluronic acid receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LYVE1, check out the LYVE1 Infographic

LYVE1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LYVE1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0967 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Du Lc, Chen Xc, Wang D, Wen Yj, Wang Ct, Wang Xm, Kan B, Wei Yq, Zhao X. Reprod Biol Endocrinol. 2014 Feb 6;12:14. Doi: 10.1186/1477-7827-12-14. Vegf-D-Induced Draining Lymphatic Enlargement And Tumor Lymphangiogenesis Promote Lymph Node Metastasi...

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6 Customer Q&As for Human LYVE-1 ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-11-24

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-11-24

Question

Can EK0967 be used to detect LYVE-1 in Human urine samples?

Verified customer

Asked: 2020-10-23

Answer

Our lab technicians have not validated the Human LYVE-1 ELISA Kit PicoKine EK0967 with urine samples, so we cannot guarantee that it would work. In theory, the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.

Boster Scientific Support

Answered: 2020-10-23

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

H. Adams

Verified customer

Asked: 2019-06-07

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-06-07

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis LYVE-1 using the EK0967 Human LYVE-1 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-04-09

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2019-04-09

Question

Q: can you suggest the dilution ratio of serum samples for detection of LYVE-1 in Human cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

D. Rodriguez

Verified customer

Asked: 2018-06-04

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the LYVE-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-06-04

Question

Q: if the enzyme conjugated LYVE-1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the LYVE-1 antigen?

Verified Customer

Verified customer

Asked: 2016-04-10

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-04-10

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