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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and cell lysates.|
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Human LYVE-1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human LYVE-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human LYVE1 in cell culture supernatants and cell lysates. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LYVE-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0967)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: S24-T238
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0967 is reactive to LYVE1 in Human samples
Validated Sample Types
cell culture supernatants and cell lysates.
EK0967 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of LYVE-1
Extracellular link domain containing 1, also known as LYVE-1 or XLKD1 is a human gene. The International Radiation Hybrid Mapping Consortium mapped the LYVE1 gene to chromosome 11p15.4. This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. This gene may play a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). It may act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
|EK0967-CAP||Anti-Human LYVE1 Pre-coated 96-well strip microplate||1|
|EK0967-ST||Human LYVE1 Standard||2 vials, 10 ng/tube|
|EK0967-DA||Human LYVE1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human LYVE-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect LYVE-1, Dilution ratio of 1:200, concentration in serum is around 50ng/ml.
Some articles we found to cite concentrations of LYVE-1 in samples: 24665348 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LYVE-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of LYVE-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For LYVE1 (Source: Uniprot.Org, NCBI)
Lymphatic vessel endothelial hyaluronic acid receptor 1
cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; LYVE-1XLKD1; XLKD1 LYVE1 CRSBP-1, HAR, LYVE-1, XLKD1 lymphatic vessel endothelial hyaluronan receptor 1 lymphatic vessel endothelial hyaluronic acid receptor 1|cell surface retention sequence binding protein-1|extracellular link domain-containing 1|extracellular link domain-containing protein 1|hyaluronic acid receptor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on LYVE1, check out the LYVE1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LYVE1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0967 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Du Lc, Chen Xc, Wang D, Wen Yj, Wang Ct, Wang Xm, Kan B, Wei Yq, Zhao X. Reprod Biol Endocrinol. 2014 Feb 6;12:14. Doi: 10.1186/1477-7827-12-14. Vegf-D-Induced Draining Lymphatic Enlargement And Tumor Lymphangiogenesis Promote Lymph Node Metastasi...
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