Pig porcine TIMP-3 ELISA Kit PicoKine®

TIMP-3 ELISA kit for Pig

Pig porcine TIMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Pig TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml.

Product Info Summary

SKU: EK0523-PO
Size: 96 wells/kit, with removable strips.
Reactive Species: Pig
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Product Name

Pig porcine TIMP-3 ELISA Kit PicoKine®

View all TIMP-3 ELISA kits

SKU/Catalog Number

EK0523-PO

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Pig porcine TIMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Pig TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Pig porcine TIMP-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0523-PO)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: C24-P211

Sensitivity

<2 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0523-PO for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0523-PO is reactive to TIMP3 in Pig samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Application Guarantee

EK0523-PO is guaranteed for ELISA in Pig by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TIMP-3

The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD).

Kit Components

Catalog Number Description Quantity
EK0523-PO-CAP Anti-Pig TIMP3 Pre-coated 96-well strip microplate 1
EK0523-PO-ST Pig TIMP3 Standard 2 vials, 10 ng/tube
EK0523-PO-DA Pig TIMP3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0190.0800.1180.2190.4370.8081.5712.420

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Pig porcine TIMP-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)125026875353143028325657
Standard deviation72.5110.2192.795.81147.3277.2
CV (%)5.8%4.1%3.6%6.7%5.2%4.9%

Gene/Protein Information For TIMP3 (Source: Uniprot.Org, NCBI)

Gene Name

TIMP3

Full Name

Metalloproteinase inhibitor 3

Weight

24.145kDa

Superfamily

protease inhibitor I35 (TIMP) family

Alternative Names

HSMRK222; K222; K222TA2; metalloproteinase inhibitor 3; MIG-5 protein; Protein MIG-5; pseudoinflammatory); SFD; TIMP metallopeptidase inhibitor 3; TIMP3; TIMP-3; tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy; Tissue inhibitor of metalloproteinases 3 TIMP3 HSMRK222, K222, K222TA2, SFD TIMP metallopeptidase inhibitor 3 metalloproteinase inhibitor 3|MIG-5 protein|TIMP-3|protein MIG-5|tissue inhibitor of metalloproteinases 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TIMP3, check out the TIMP3 Infographic

TIMP3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TIMP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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9 Customer Q&As for Pig porcine TIMP-3 ELISA Kit PicoKine®

Question

Q: which procedure should I follow in order to thaw whole blood sample for TIMP-3 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-11-30

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TIMP-3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-11-30

Question

Q: can I use citrate plasma as samples in Pig TIMP-3 Picokine® ELISA Kit (Catalog # EK0523-PO)?

K. Gonzalez

Verified customer

Asked: 2020-11-28

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TIMP-3 causing disruption of its protein structure. TIMP-3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TIMP-3 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-11-28

Question

Q: if the enzyme conjugated TIMP-3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TIMP-3 antigen?

W. Liu

Verified customer

Asked: 2019-07-29

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-07-29

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-07-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-07-22

Question

Q: how to analyze ELISA data? I measured TIMP-3 level in serum.

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-06-10

Question

Q: What is the optimal O.D. value for TIMP-3 ELISA kit? I performed your TIMP-3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TIMP-3 even though the O.D. values are not very high?

K. Smith

Verified customer

Asked: 2018-12-12

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TIMP-3 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of TIMP-3 that can be declared/interpreted as positive by the above standard is 2pg/ml.

Boster Scientific Support

Answered: 2018-12-12

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-09-17

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-09-17

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

H. Brown

Verified customer

Asked: 2018-05-10

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-05-10

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2016-10-13

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-10-13

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