Anti-VEGF Receptor 1/FLT1 Antibody Picoband®

FLT1 antibody

Boster Bio Anti-VEGF Receptor 1/FLT1 Antibody catalog # PA1399. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PA1399
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-VEGF Receptor 1/FLT1 Antibody Picoband®

View all FLT1 Antibodies

SKU/Catalog Number

PA1399

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-VEGF Receptor 1/FLT1 Antibody catalog # PA1399. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VEGF Receptor 1/FLT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1399)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human FLT1.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1399 is reactive to FLT1 in Human

Observed Molecular Weight

151 kDa

Calculated molecular weight

150.8 kDa

Background of FLT1

Vascular endothelial growth factor receptor 1 (FLT1) is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family. It is mapped to 13q12. The deduced 1,338-amino acid protein has a calculated molecular mass of 150.6 kD. It has a 758-amino acid extracellular domain, followed by a 22-amino acid transmembrane region and a 558-amino acid cytoplasmic region containing a cluster of basic amino acids and a tyrosine kinase domain. sFLT-1 was identified in placenta, adult lung, kidney, liver and uterus. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1399 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: MCF-7 Cell, SGC Cell, MM231 Cell

Validation Images & Assay Conditions

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6 Customer Q&As for Anti-VEGF Receptor 1/FLT1 Antibody Picoband®

Question

Will anti-VEGF Receptor 1/FLT1 antibody PA1399 work on bovine WB with umbilical vein?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

Our lab technicians have not tested anti-VEGF Receptor 1/FLT1 antibody PA1399 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-VEGF Receptor 1/FLT1 antibody PA1399 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine umbilical vein in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-11

Question

Would PA1399 anti-VEGF Receptor 1/FLT1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

As indicated on the product datasheet, PA1399 anti-VEGF Receptor 1/FLT1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-17

Question

I have a question about product PA1399, anti-VEGF Receptor 1/FLT1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-06-28

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1399 anti-VEGF Receptor 1/FLT1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-06-28

Question

We are interested in to test anti-VEGF Receptor 1/FLT1 antibody PA1399 on human umbilical vein for research purposes, then I may be interested in using anti-VEGF Receptor 1/FLT1 antibody PA1399 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-11-08

Answer

The products we sell, including anti-VEGF Receptor 1/FLT1 antibody PA1399, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-11-08

Question

Would anti-VEGF Receptor 1/FLT1 antibody PA1399 work for WB with umbilical vein?

J. Zhao

Verified customer

Asked: 2015-03-18

Answer

According to the expression profile of umbilical vein, FLT1 is highly expressed in umbilical vein. So, it is likely that anti-VEGF Receptor 1/FLT1 antibody PA1399 will work for WB with umbilical vein.

Boster Scientific Support

Answered: 2015-03-18

Question

I see that the anti-VEGF Receptor 1/FLT1 antibody PA1399 works with WB, what is the protocol used to produce the result images on the product page?

H. Edwards

Verified customer

Asked: 2014-02-28

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com

Boster Scientific Support

Answered: 2014-02-28

Order DetailsPrice
PA1399

100μg

$370
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100 μg Fluoro550 conjugated (liquid)

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100 μg Fluoro594 conjugated (liquid)

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PA1399-Fluoro647

100 μg Fluoro647 conjugated (liquid)

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PA1399-APC

100 μg APC conjugated (liquid)

$820
PA1399-FITC

100 μg FITC conjugated (liquid)

$570
PA1399-Cy3

100 μg Cy3 conjugated (liquid)

$570
PA1399-Biotin

100 μg Biotin conjugated (liquid)

$570
PA1399-HRP

100 μg HRP conjugated (liquid)

$570
PA1399-PE

100 μg PE conjugated (liquid)

$820
PA1399-carrier-free

Carrier Free

$370
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Free Secondary Antibody

FREE  $95.00
FREE  $95.00
$40.00  $80.00
$65.00  $130.00
$67.50  $135.00
$75.00  $150.00
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PA1399
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Apr 12, 2026