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- Table of Contents
VIL1 antibody (PB9457) performs well in Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Flow Cytometry analysis of CACO-2 cells using anti-Villin antibody (PB9457).
Overlay histogram showing CACO-2 cells stained with PB9457 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Villin Antibody (PB9457, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing CACO-2 cells stained with PB9457 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Villin Antibody (PB9457, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Villin/VIL1 Antibody Picoband®
VIL1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, WB |
Tested Applications
VIL1 antibody was positively detected by WB in:
Rat Intestine Tissue, Mouse Kidney Tissue, RH35 Whole Cell, HEPG2 Whole Cell, MCF-7 Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
VIL1 antibody was positively detected by IHC in:
mouse intestine tissue, rat intestine tissue, human intestinal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
VIL1 antibody was positively detected by IF in:
human rectal cancer tissue, rat intestine tissue, mouse intestine tissue, human ileum tissue, human colon organoid tissue, mouse ileum tissue, mouse ileum organoid tissue
VIL1 antibody was positively detected by FC in:
CACO-2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunofluorescence
2μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Human
Tested
Tested
Monkey
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Villin/VIL1 Antibody Picoband®
View all VIL1 Antibodies
View all VIL1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Villin, different from the related mouse sequence by three amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
93 kDa
Calculated Molecular Weight
92.7 kDa
Background of VIL1
Villin is known as VIL1. This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. In vertebrates, the villin proteins help to support the microfilaments of the microvilli of the brush border. It may play a role in cell plasticity through F-actin severing.
Cite This Product
Anti-Villin/VIL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9457)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rat
93%
High Homology
Cow
93%
High Homology
Mouse
90%
High Homology
Sheep
90%
High Homology
Dog
90%
High Homology
Rabbit
87%
Moderate Homology
Zebrafish
63%
Low homology
Drosophila
30%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for VIL1 antibody
Immunohistochemistry (IHC) protocol for VIL1 antibody
Immunofluorescence (IF) protocol for VIL1 antibody
Flow Cytometry (FC) protocol for VIL1 antibody
Publications
Application:
Species:
Senecavirus a can replicate in apical-out porcine intestinal organoids and induce stress granules and innate immune response
Published: 2025-08-24
Journal: Virulence
Application: IF
Impact Factor: 5.40
Species: Porcine
PMID: 40849899
Gasdermin-D-mediated epithelial-immune circuit synchronizes nutrient absorption and host defense in the small intestine
Published: 2025-07-24
Journal: IMMUNITY
Application: IHC,IF,WB
Impact Factor: 26.30
Species: Mouse
PMID: 40712560
Therapeutic Impact of Polysaccharide from Chinese Yam (Dioscorea opposita Thunb) on DSS-Induced Ulcerative Colitis in Mice
Published: 2025-06-11
Journal: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Application: IF
Impact Factor: 1.30
Species: Mouse
PMID: 40498533
Prevention and Alleviation of Dextran Sulfate Sodium Salt-Induced Inflammatory Bowel Disease in Mice With Bacillus subtilis-Fermented Milk via Inhibition of the Inflammatory Responses and Regulation of the Intestinal Flora
Published: 2021-01-07
Journal: Frontiers in Microbiology
Application: WB
Impact Factor: 5.64
Species: Mouse
PMID: 33519783
Acid and bile salt up-regulate BMP4 expression in human esophageal epithelium cells
Published: 2009-08-13
Journal: SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
Application: WB
Impact Factor: 1.98
Species: Human
PMID: 19488929
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Product Promise
We promise Anti-Villin/VIL1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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PB9457
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026