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Product Info Summary
| SKU: | M00210 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
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Product info
Product Name
Anti-Vitamin D Receptor VDR Rabbit Monoclonal Antibody
SKU/Catalog Number
M00210
BM5055 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Vitamin D Receptor VDR Rabbit Monoclonal Antibody catalog # M00210. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vitamin D Receptor VDR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00210)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAGE-22
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Vitamin D Receptor
Reactive Species
M00210 is reactive to VDR in Human, Mouse, Rat
Observed Molecular Weight
48 kDa
Calculated molecular weight
48.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00210 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:50
Validation Images & Assay Conditions
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Western blot analysis of VDR using anti-VDR antibody (M00210).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VDR antigen affinity purified monoclonal antibody (Catalog # M00210) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VDR at approximately 48 kDa. The expected band size for VDR is at 48 kDa.
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Specific Publications For Anti-Vitamin D Receptor VDR Rabbit Monoclonal Antibody (M00210)
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4 Customer Q&As for Anti-Vitamin D Receptor VDR Rabbit Monoclonal Antibody
Question
I would like using your anti-Vitamin D Receptor Rabbit Monoclonal antibody for skeletal system development studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
We appreciate your inquiry. This M00210 anti-Vitamin D Receptor Rabbit Monoclonal antibody is tested on hela cell lysate, mouse kidney. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-17
Question
We have observed staining in rat placenta. Are there any suggestions? Is anti-Vitamin D Receptor Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
From what I have seen in literature placenta does express VDR. From what I have seen in Uniprot.org, VDR is expressed in tibia, lens epithelium, placenta, peripheral blood, prostate, among other tissues. Regarding which tissues have VDR expression, here are a few articles citing expression in various tissues:
Lens epithelium, Pubmed ID: 9212063
Peripheral blood, Pubmed ID: 1850412, 16252240
Placenta, Pubmed ID: 15489334
Prostate, Pubmed ID: 22323358
Boster Scientific Support
Answered: 2019-12-31
Question
My colleagues were well pleased with the WB result of your anti-Vitamin D Receptor Rabbit Monoclonal antibody. However we have observed positive staining in tibia nucleus using this antibody. Is that expected? Could you tell me where is VDR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-04-16
Answer
From literature, tibia does express VDR. Generally VDR expresses in nucleus. Regarding which tissues have VDR expression, here are a few articles citing expression in various tissues:
Lens epithelium, Pubmed ID: 9212063
Peripheral blood, Pubmed ID: 1850412, 16252240
Placenta, Pubmed ID: 15489334
Prostate, Pubmed ID: 22323358
Boster Scientific Support
Answered: 2018-04-16
Question
We are currently using anti-Vitamin D Receptor Rabbit Monoclonal antibody M00210 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
F. Collins
Verified customer
Asked: 2015-01-23
Answer
The anti-Vitamin D Receptor Rabbit Monoclonal antibody (M00210) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-23
