Anti-FGF23 Antibody Picoband™

Boster Bio Anti-FGF23 Antibody Picoband™ catalog # A00478-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: A00478-3
Size: 100μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-FGF23 Antibody Picoband™

See all FGF-23 products

SKU/Catalog Number

A00478-3

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-FGF23 Antibody Picoband™ catalog # A00478-3. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FGF23 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00478-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived rat FGF23 recombinant protein (Position: Y25-V251).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00478-3 is reactive to Fgf23 in Mouse, Rat

Applications

A00478-3 is guaranteed for ELISA, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Fgf23 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8VI82

Gene ID

170583

Gene Name

Fgf23

Full Name

Fibroblast growth factor 23

Weight

27.912kDa

Superfamily

heparin-binding growth factors family

Alternative Names

ADHR; FGF23; FGF-23; fibroblast growth factor 23; HPDR2; HYPF; phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor Fgf23|Fgf8b|fibroblast growth factor 23|fibroblast growth factor 23|FGF-23|fibroblast growth factor 8b

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Fgf23, check out the Fgf23 Infographic

Fgf23 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Fgf23: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A00478-3

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Find answers in Q&As, reviews.

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15 Customer Q&As for Anti-FGF23 Antibody Picoband™

Question

Is a blocking peptide available for product anti-FGF23 antibody (A00478-3)?

G. Dhar

Verified customer

Asked: 2020-01-28

Answer

We do provide the blocking peptide for product anti-FGF23 antibody (A00478-3). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-28

Question

Will anti-FGF23 antibody A00478-3 work on monkey WB with right atrium auricular region?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

Our lab technicians have not tested anti-FGF23 antibody A00478-3 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-FGF23 antibody A00478-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey right atrium auricular region in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-13

Question

I was wanting to use to test anti-FGF23 antibody A00478-3 on rat right atrium auricular region for research purposes, then I may be interested in using anti-FGF23 antibody A00478-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

The products we sell, including anti-FGF23 antibody A00478-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-06

Question

I was wanting to use your anti-FGF23 antibody for ELISA for rat right atrium auricular region on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat right atrium auricular region identification?

Verified Customer

Verified customer

Asked: 2019-11-08

Answer

You can see on the product datasheet, A00478-3 anti-FGF23 antibody has been validated for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat right atrium auricular region in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-08

Question

Please see the WB image, lot number and protocol we used for right atrium auricular region using anti-FGF23 antibody A00478-3. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-24

Question

My question regarding product A00478-3, anti-FGF23 antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00478-3 anti-FGF23 antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-08

Question

Is this A00478-3 anti-FGF23 antibody reactive to the isotypes of FGF23?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

The immunogen of A00478-3 anti-FGF23 antibody is E. coli-derived rat FGF23 recombinant protein (Position: Y25-V251). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-03

Question

Will A00478-3 anti-FGF23 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

It shows on the product datasheet, A00478-3 anti-FGF23 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-24

Question

Does anti-FGF23 antibody A00478-3 work for ELISA with right atrium auricular region?

Verified Customer

Verified customer

Asked: 2019-01-29

Answer

According to the expression profile of right atrium auricular region, FGF23 is highly expressed in right atrium auricular region. So, it is likely that anti-FGF23 antibody A00478-3 will work for ELISA with right atrium auricular region.

Boster Scientific Support

Answered: 2019-01-29

Question

We would like to know if these three products can be used in IHC on rats: https://www.bosterbio.com/anti-fgf23-picoband-trade-antibody-a00478-3-boster.html#gene-info https://www.bosterbio.com/anti-nr3c2-picoband-trade-antibody-pb9765-boster.html?options=cart https://www.bosterbio.com/anti-nr3c2-antibody-pa1594-boster.html?options=cart

Verified Customer

Verified customer

Asked: 2018-03-13

Answer

A00478-3, PB9765, and PA1594 can not be used for IHC on rats.

Boster Scientific Support

Answered: 2018-03-13

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right atrium auricular region using anti-FGF23 antibody A00478-3. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-01-29

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-29

Question

We are currently using anti-FGF23 antibody A00478-3 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-11-01

Answer

The anti-FGF23 antibody (A00478-3) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-11-01

Question

I am interested in using your anti-FGF23 antibody for cell differentiation studies. Has this antibody been tested with western blotting on rat testis tissue? We would like to see some validation images before ordering.

C. Dhar

Verified customer

Asked: 2015-11-30

Answer

Thanks for your inquiry. This A00478-3 anti-FGF23 antibody is validated on rat testis tissue, tissue lysate, mouse testis tissue. It is guaranteed to work for ELISA, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-11-30

Question

I see that the anti-FGF23 antibody A00478-3 works with ELISA, what is the protocol used to produce the result images on the product page?

M. Krishna

Verified customer

Asked: 2015-04-01

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-04-01

Question

Do you have a BSA free version of anti-FGF23 antibody A00478-3 available?

A. Brown

Verified customer

Asked: 2014-08-18

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FGF23 antibody A00478-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-08-18

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A00478-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

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