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Product Info Summary
| SKU: | M03552 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-XLF (NHEJ1) Mouse Monoclonal Antibody [Clone ID: OTI3D6]
SKU/Catalog Number
M03552
Size
100 µl
Description
Boster Bio NHEJ1 (XLF) mouse monoclonal antibody, clone OTI3D6 (formerly 3D6). Catalog# M03552. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-XLF (NHEJ1) Mouse Monoclonal Antibody [Clone ID: OTI3D6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03552)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3D6
Isotype
IgG1
Immunogen
Full length human recombinant protein of human NHEJ1 (NP_079058) produced in HEK293T cell.
Reactive Species
M03552 is reactive to NHEJ1 in Human, Monkey
Calculated molecular weight
33.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03552 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000~2000
IHC 1:150
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NHEJ1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NHEJ1 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Flow cytometric Analysis of Hela cells
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Anti-NHEJ1 mouse monoclonal antibody (M03552) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NHEJ1.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and NHEJ1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1 monoclonal antibody M03552 (1:200). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Specific Publications For Anti-XLF (NHEJ1) Mouse Monoclonal Antibody [Clone ID: OTI3D6] (M03552)
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