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Product Info Summary
| SKU: | A00568 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HLA Class II DRB1/HLA-DRB1 Antibody Picoband®
SKU/Catalog Number
A00568
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HLA Class II DRB1/HLA-DRB1 Antibody Picoband® catalog # A00568. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLA Class II DRB1/HLA-DRB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00568)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HLA Class II DRB1/HLA-DRB1.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00568 is reactive to HLA-DRB1 in Human
Observed Molecular Weight
25-30 kDa
Calculated molecular weight
30.0 kDa
Background of HLA-DRB1
HLA class II histocompatibility antigen, DRB1 beta chain is a protein that in humans is encoded by the HLA-DRB1 gene. This gene is mapped to 6p21.32. DRB1 encodes the most prevalent beta subunit of HLA-DR. Several alleles of DRB1 (shared epitope alleles) are associated with an increased incidence of rheumatoid arthritis. HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. It plays a central role in the immune system by presenting peptides derived from extracellular proteins to T helper cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00568 is guaranteed for IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.1-0.5μg/ml | Human |
| Immunohistochemistry (Paraffin-embedded Section) | 2-5μg/ml | Human |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Validation Images & Assay Conditions
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Western blot analysis of HLA-DRB1 using anti-HLA-DRB1 antibody (A00568).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human HUT78 whole cell lysates,
Lane 4: human Ramos whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DRB1 antigen affinity purified polyclonal antibody (A00568) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HLA-DRB1 at approximately 25-30 kDa. The expected band size for HLA-DRB1 is at 30 kDa.
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IHC analysis of HLA-DRB1 using anti-HLA-DRB1 antibody (A00568).
HLA-DRB1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HLA-DRB1 Antibody (A00568) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-HLA Class II DRB1/HLA-DRB1 Antibody Picoband® (A00568)
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Customer Q&As
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1 Customer Q&As for Anti-HLA Class II DRB1/HLA-DRB1 Antibody Picoband®
Question
We are currently using anti-HLA Class II DRB1/HLA-DRB1 antibody A00568 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The anti-HLA Class II DRB1/HLA-DRB1 antibody (A00568) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-10
