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Product Info Summary
| SKU: | A01182-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-XPA Antibody
SKU/Catalog Number
A01182-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-XPA Antibody catalog # A01182-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-XPA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01182-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human XPA. AA range:211-260
Reactive Species
A01182-1 is reactive to XPA in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
31.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01182-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using XPA Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from COLO205 cells, using XPA Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using XPA Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-XPA Antibody (A01182-1)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-XPA Antibody
Question
I have a question about product A01182-1, anti-XPA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01182-1 anti-XPA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-20
Question
I am interested in to test anti-XPA antibody A01182-1 on mouse b-cell for research purposes, then I may be interested in using anti-XPA antibody A01182-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
The products we sell, including anti-XPA antibody A01182-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-26
Question
Is a blocking peptide available for product anti-XPA antibody (A01182-1)?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
We do provide the blocking peptide for product anti-XPA antibody (A01182-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-13
Question
Is this A01182-1 anti-XPA antibody reactive to the isotypes of XPA?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
The immunogen of A01182-1 anti-XPA antibody is Synthesized peptide derived from the C-terminal region of human XPA. at AA rangle: 180-260. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-30
Question
I see that the anti-XPA antibody A01182-1 works with WB, what is the protocol used to produce the result images on the product page?
M. Gonzalez
Verified customer
Asked: 2016-10-31
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-10-31
Question
Would anti-XPA antibody A01182-1 work on primate WB with spermatocyte?
P. Li
Verified customer
Asked: 2015-01-20
Answer
Our lab technicians have not tested anti-XPA antibody A01182-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-XPA antibody A01182-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate spermatocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-20
Question
Would anti-XPA antibody A01182-1 work for WB with b-cell?
C. Baker
Verified customer
Asked: 2014-03-17
Answer
According to the expression profile of b-cell, XPA is highly expressed in b-cell. So, it is likely that anti-XPA antibody A01182-1 will work for WB with b-cell.
Boster Scientific Support
Answered: 2014-03-17
