Anti-HAUSP/USP7 Antibody Picoband™ (monoclonal, 5E2)

Boster Bio Anti-HAUSP/USP7 Antibody Picoband™ (monoclonal, 5E2) catalog # M01239-1. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01239-1
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-HAUSP/USP7 Antibody Picoband™ (monoclonal, 5E2)

See all USP7 products

SKU/Catalog Number

M01239-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HAUSP/USP7 Antibody Picoband™ (monoclonal, 5E2) catalog # M01239-1. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HAUSP/USP7 Antibody Picoband™ (monoclonal, 5E2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01239-1)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

5.00E+02

Isotype

IgG2b

Immunogen

E.coli-derived human HAUSP/USP7 recombinant protein (Position: L258-D483).

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M01239-1 is reactive to USP7 in Human, Mouse, Rat

Applications

M01239-1 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For USP7 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q93009

Gene ID

7874

Gene Name

USP7

Full Name

Ubiquitin carboxyl-terminal hydrolase 7

Weight

128.302kDa

Superfamily

peptidase C19 family

Alternative Names

Deubiquitinating enzyme 7; EC 3.1.2.15; EC 3.4.19.12; HAUSP; HAUSPTEF1; Herpes virus-associated ubiquitin-specific protease; Herpesvirus-associated ubiquitin-specific protease; TEF1; ubiquitin carboxyl-terminal hydrolase 7; ubiquitin specific peptidase 7 (herpes virus-associated); ubiquitin specific protease 7 (herpes virus-associated); Ubiquitin thioesterase 7; Ubiquitin-specific-processing protease 7; USP7 USP7 HAFOUS, HAUSP, TEF1 ubiquitin specific peptidase 7 ubiquitin carboxyl-terminal hydrolase 7|Herpes virus-associated ubiquitin-specific protease|deubiquitinating enzyme 7|ubiquitin specific peptidase 7 (herpes virus-associated)|ubiquitin specific protease 7 (herpes virus-associated)|ubiquitin thioesterase 7|ubiquitin-specific-processing protease 7

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on USP7, check out the USP7 Infographic

USP7 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for USP7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Anti-HAUSP/USP7 Antibody Picoband™ (monoclonal, 5E2)

Question

Would M01239-1 anti-HAUSP/USP7 antibody (monoclonal, 5E2) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

It shows on the product datasheet, M01239-1 anti-HAUSP/USP7 antibody (monoclonal, 5E2) as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-24

Question

Does anti-HAUSP/USP7 antibody (monoclonal, 5E2) M01239-1 work on pig IF with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2018-11-20

Answer

Our lab technicians have not tested anti-HAUSP/USP7 antibody (monoclonal, 5E2) M01239-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-HAUSP/USP7 antibody (monoclonal, 5E2) M01239-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-20

Question

Is there a BSA free version of anti-HAUSP/USP7 antibody (monoclonal, 5E2) M01239-1 available?

Verified Customer

Verified customer

Asked: 2018-06-18

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-HAUSP/USP7 antibody (monoclonal, 5E2) M01239-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-06-18

Question

We are currently using anti-HAUSP/USP7 antibody (monoclonal, 5E2) M01239-1 for rat tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2017-10-04

Answer

The anti-HAUSP/USP7 antibody (monoclonal, 5E2) (M01239-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-10-04

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M01239-1
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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