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Product Info Summary
| SKU: | M00571 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-XRCC1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00571
BM5088 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-XRCC1 Rabbit Monoclonal Antibody catalog # M00571. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-XRCC1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00571)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABOH-24
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human XRCC1
Reactive Species
M00571 is reactive to XRCC1 in Human, Mouse, Rat
Observed Molecular Weight
90 kDa
Calculated molecular weight
69.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00571 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: HeLa cell
IHC: human testis tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of XRCC1 using anti-XRCC1 antibody (M00571).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC1 antigen affinity purified monoclonal antibody (M00571) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for XRCC1 at approximately 90 kDa. The expected band size for XRCC1 is at 69 kDa.
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Immunohistochemical analysis of paraffin-embedded human testis, using XRCC1 Antibody.
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Immunofluorescent analysis of Hela cells, using XRCC1 Antibody .
Specific Publications For Anti-XRCC1 Rabbit Monoclonal Antibody (M00571)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-XRCC1 Rabbit Monoclonal Antibody
Question
We bought anti-XRCC1 Rabbit Monoclonal antibody for WB on leukemic t-cell a few years ago. I am using human, and We want to use the antibody for IF next. We want examining leukemic t-cell as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
I took a look at the website and datasheets of our anti-XRCC1 Rabbit Monoclonal antibody and it seems that M00571 has been tested on human in both WB and IF. Thus M00571 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-01
Question
I see that the anti-XRCC1 Rabbit Monoclonal antibody M00571 works with WB, what is the protocol used to produce the result images on the product page?
K. Krishna
Verified customer
Asked: 2020-03-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-03-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-XRCC1 Rabbit Monoclonal antibody M00571. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-16
Question
Do you have a BSA free version of anti-XRCC1 Rabbit Monoclonal antibody M00571 available?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-XRCC1 Rabbit Monoclonal antibody M00571 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-14
Question
Will M00571 anti-XRCC1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
As indicated on the product datasheet, M00571 anti-XRCC1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-25
Question
Our lab were content with the WB result of your anti-XRCC1 Rabbit Monoclonal antibody. However we have seen positive staining in eye nucleus using this antibody. Is that expected? Could you tell me where is XRCC1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
According to literature, eye does express XRCC1. Generally XRCC1 expresses in nucleus. Regarding which tissues have XRCC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-11-22
Question
We are currently using anti-XRCC1 Rabbit Monoclonal antibody M00571 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The anti-XRCC1 Rabbit Monoclonal antibody (M00571) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-16
Question
Is a blocking peptide available for product anti-XRCC1 Rabbit Monoclonal antibody (M00571)?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
We do provide the blocking peptide for product anti-XRCC1 Rabbit Monoclonal antibody (M00571). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-04
Question
I was wanting to use your anti-XRCC1 Rabbit Monoclonal antibody for WB for human eye on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human eye identification?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
It shows on the product datasheet, M00571 anti-XRCC1 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human eye in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
Here is the WB image, lot number and protocol we used for eye using anti-XRCC1 Rabbit Monoclonal antibody M00571. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-01
Question
I have a question about product M00571, anti-XRCC1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Bhatt
Verified customer
Asked: 2019-05-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00571 anti-XRCC1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-10
Question
Is this M00571 anti-XRCC1 Rabbit Monoclonal antibody reactive to the isotypes of XRCC1?
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
The immunogen of M00571 anti-XRCC1 Rabbit Monoclonal antibody is A synthesized peptide derived from human XRCC1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-03
Question
I was wanting to use to test anti-XRCC1 Rabbit Monoclonal antibody M00571 on human eye for research purposes, then I may be interested in using anti-XRCC1 Rabbit Monoclonal antibody M00571 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
The products we sell, including anti-XRCC1 Rabbit Monoclonal antibody M00571, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-01
Question
Does anti-XRCC1 Rabbit Monoclonal antibody M00571 work for WB with eye?
Verified Customer
Verified customer
Asked: 2018-01-08
Answer
According to the expression profile of eye, XRCC1 is highly expressed in eye. So, it is likely that anti-XRCC1 Rabbit Monoclonal antibody M00571 will work for WB with eye.
Boster Scientific Support
Answered: 2018-01-08
Question
We have seen staining in mouse cervix carcinoma erythroleukemia. Any tips? Is anti-XRCC1 Rabbit Monoclonal antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2017-05-18
Answer
From what I have seen in literature cervix carcinoma erythroleukemia does express XRCC1. From what I have seen in Uniprot.org, XRCC1 is expressed in right ovary, eye, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have XRCC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2017-05-18
Question
We are interested in using your anti-XRCC1 Rabbit Monoclonal antibody for negative regulation of protein adp-ribosylation studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
K. Anderson
Verified customer
Asked: 2017-04-25
Answer
I appreciate your inquiry. This M00571 anti-XRCC1 Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-04-25
