ACOT8 Antibodies

AND ACOT8 ELISA kits, ACOT8 Proteins

Many biological assays use antibodies to detect ACOT8, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with ACOT8 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop ACOT8 antibodies...

We validate the specificity of these antibodies to ACOT8 by testing them on tissues known to express ACOT8 positively and negatively. Browse below to find the ACOT8 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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ACOT8 Infographic by Boster Bio

All ACOT8 Products

Product

Figures

Specificity

Applications

Price

Anti-Acyl-coenzyme A thioesterase 8 ACOT8 Antibody

5 Q&As

Human, Mouse, Rat

IF, WB

$360
Cat # A09392
100 µl

Anti-Acyl-coenzyme A thioesterase 8 ACOT8 Antibody

Human

ELISA, IF, IHC, WB

$380
Cat # A09392-1
100ul

Human ACOT8 Recombinant Protein

SDS-PAGE

$250
Cat # PROTO14734
Starting from 20ug

Acyl coenzyme A Thioesterase 8 (ACOT8) (NM_005469) Human Over-expression Lysate

Human

$628
Cat # LS010005
100 µg

ACOT8 Overview

Facts about Acyl-coenzyme A thioesterase 8.

ACOT8 3D structure. Source: alphafold

Acyl-coenzyme A thioesterase 8 (ACOT8)

Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:9299485, PubMed:9153233, PubMed:15194431). Competes with bile acid CoA:amino acid N-acyltransferase (BAAT) for bile acid-CoA substrate (for example, chenodeoxycholoyl-CoA).

Shows a preference for medium-length fatty acyl-CoAs (C2 to C20) (PubMed:9299485, PubMed:9153233). Inactive towards substrates with more than C20 aliphatic chains (PubMed:9153233).

Gene Information

Human
Gene Name:	ACOT8
Uniprot:	O14734
Entrez:	10005

Family

Belongs to:

C/M/P thioester hydrolase family

Alternative Names

ACTEIII; acyl-CoA thioesterase 8HNAACTE; Choloyl-coenzyme A thioesterase; EC 3.1.2.27; hACTEIII; HIV-Nef-associated acyl-CoA thioesterase; hTEcholoyl-CoA hydrolase; long-chain fatty-acyl-CoA hydrolase; palmitoyl-CoA hydrolase; peroxisomal acyl-CoA thioesterase 1; peroxisomal acyl-CoA thioesterase; Peroxisomal acyl-coenzyme A thioester hydrolase 1; Peroxisomal long-chain acyl-CoA thioesterase 1; PTE-1; PTE1HIV-Nef associated acyl-CoA thioesterase; PTE2; PTE-2hACTE-IIIacyl-coenzyme A thioesterase 8; Thioesterase II; thioesterase III

Molecular Weight

Mass (kDA):
35.914 kDA

Genomic Context


Human

Location:	20q13.12
Sequence:	20; NC_000020.11 (45841721..45857405, complement)

Tissue Specificity

Detected in a T-cell line (at protein level). Ubiquitous (PubMed:9153233, PubMed:9299485).

Subcellular Localizations

Peroxisome matrix. Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded.

Diseases

Immunologic Deficiency Syndromes
Contagious Equine Metritis
Hiv Infections

Interacting Proteins

PEX5
REL

Pathways

Cell Activation
T Cell Activation
Fatty Acid Oxidation
Localization

PTMs

Oxidation
Glycosylation

Amidation
Palmitoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ACOT8

References

PMID: 9299485 by Watanabe H., et al. A novel acyl-CoA thioesterase enhances its enzymatic activity by direct binding with HIV Nef.

PMID: 9153233 by Liu L.X., et al. Binding of HIV-1 Nef to a novel thioesterase enzyme correlates with Nef-mediated CD4 down-regulation.