This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
ACOT8 Antibodies
AND ACOT8 ELISA kits, ACOT8 Proteins
Many biological assays use antibodies to detect ACOT8, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with ACOT8 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop ACOT8 antibodies...
Designing an IHC experiment for ACOT8? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the ACOT8 IHC GuideACOT8 Infographic by Boster Bio
All ACOT8 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, IF, IHC, WB
$380
Cat # A09392-1
100ul
Cat # A09392-1
100ul
Human
$628
Cat # LS010005
20 µg, 100 µg
Cat # LS010005
20 µg, 100 µg
ACOT8 Overview
Facts about Acyl-coenzyme A thioesterase 8.
Acyl-coenzyme A thioesterase 8 (ACOT8)
Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:9299485, PubMed:9153233, PubMed:15194431). Competes with bile acid CoA:amino acid N-acyltransferase (BAAT) for bile acid-CoA substrate (for example, chenodeoxycholoyl-CoA).
Shows a preference for medium-length fatty acyl-CoAs (C2 to C20) (PubMed:9299485, PubMed:9153233). Inactive towards substrates with more than C20 aliphatic chains (PubMed:9153233).
Gene Information
| Human | |
|---|---|
| Gene Name: | ACOT8 |
| Uniprot: | O14734 |
| Entrez: | 10005 |
Family
| Belongs to: |
|---|
| C/M/P thioester hydrolase family |
Alternative Names
ACTEIII; acyl-CoA thioesterase 8HNAACTE; Choloyl-coenzyme A thioesterase; EC 3.1.2.27; hACTEIII; HIV-Nef-associated acyl-CoA thioesterase; hTEcholoyl-CoA hydrolase; long-chain fatty-acyl-CoA hydrolase; palmitoyl-CoA hydrolase; peroxisomal acyl-CoA thioesterase 1; peroxisomal acyl-CoA thioesterase; Peroxisomal acyl-coenzyme A thioester hydrolase 1; Peroxisomal long-chain acyl-CoA thioesterase 1; PTE-1; PTE1HIV-Nef associated acyl-CoA thioesterase; PTE2; PTE-2hACTE-IIIacyl-coenzyme A thioesterase 8; Thioesterase II; thioesterase III
Molecular Weight
Mass (kDA):
35.914 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 20q13.12 |
| Sequence: | 20; NC_000020.11 (45841721..45857405, complement) |
Tissue Specificity
Detected in a T-cell line (at protein level). Ubiquitous (PubMed:9153233, PubMed:9299485).
Subcellular Localizations
Peroxisome matrix. Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded.
Diseases
- Immunologic Deficiency Syndromes
- Contagious Equine Metritis
- Hiv Infections
Interacting Proteins
- PEX5
- REL
Pathways
- Cell Activation
- T Cell Activation
- Fatty Acid Oxidation
- Localization
PTMs
- Oxidation
- Glycosylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ACOT8
bosterbio.com/bosterbio-gene-info-cards/ACOT8
References
PMID: 9299485 by Watanabe H., et al. A novel acyl-CoA thioesterase enhances its enzymatic activity by direct binding with HIV Nef.
PMID: 9153233 by Liu L.X., et al. Binding of HIV-1 Nef to a novel thioesterase enzyme correlates with Nef-mediated CD4 down-regulation.