DKK2 Antibodies

Dickkopf-related protein 2 (DKK2)

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation.

In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity). .

Gene Information

Human
Gene Name:	DKK2
Uniprot:	Q9UBU2
Entrez:	27123

Family

Belongs to:
dickkopf family

Alternative Names

dickkopf (Xenopus laevis) homolog 2; dickkopf 2; dickkopf homolog 2 (Xenopus laevis); dickkopf related protein-2; dickkopf-2; dickkopf-related protein 2; Dkk2; Dkk-2; hDkk-2

Molecular Weight

Mass (kDA):
28.447 kDA

Genomic Context

Human
Location:	4q25
Sequence:	4; NC_000004.12 (106921802..107036313, complement)

Tissue Specificity

Expressed in heart, brain, skeletal muscle and lung.

Subcellular Localizations

Secreted.

Diseases

• Neoplasms
• Malignant Neoplasms
• Cell Transformation, Neoplastic
• Carcinoma
• Colorectal Cancer
• Malignant Paraganglionic Neoplasm
• Crest Syndrome
• Malignant Neoplasm Of Stomach
• Neoplasm Metastasis
• Inflammation
• Osteopenia
• Hypertrophy
• Neuroblastoma

• Tissue Adhesions
• Corneal Diseases
• Osteoporosis
• Malignant Neoplasm Of Gastrointestinal Tract
• Carcinogenesis
• Kidney Neoplasm
• Tumor Angiogenesis

Pathways

• Osteoblast Differentiation
• Methylation
• Cell Growth
• Cell Differentiation
• Cell Proliferation
• Regeneration
• Osteoclast Differentiation
• Cell Cycle
• Bone Resorption
• Cell Migration
• Cell Maturation
• Cell Motility
• Eye Development

• Ovulation
• Pathogenesis
• Pigmentation
• Reverse Transcription
• Cell Death
• Bone Remodeling
• Angiogenesis

PTMs

• Methylation
• Phosphorylation

• Demethylation
• Glycosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DKK2

Boster Bio Anti-DKK2 - Best Uses Of The DKK2 Marker

As a scientist, you might be wondering what to do with the Boster Bio Anti-DKK2 antibody. The antibody is an excellent tool for your research. The results you submit can lead to product credits and special samples. These product credits are available to scientists around the globe. Your results can be submitted for species, applications, or special samples. These results may be published and used in research.

Boster Bio Anti DKK2 antibody

Boster Bio Anti DKK2 antibody reacts well with DKK2 (human and mouse) and is supplied in PBS containing 0,09% sodium azide. It is purified via a protein-G column and dialysis using PBS. This antibody was produced by immunization mice with the human DKK2 Recombinant Protein. In this study, serum DKK1 concentrations were significantly higher in LN/SLE patients than in healthy controls.

A previous study demonstrated that DKK2 promotes angiogenesis in human and rodent endothelial cells. It also increased angiogenesis using a Matrigel plug assay. Anti-DKK2 antibodies also inhibit tumor angiogenesis indirectly or directly. By inhibiting DKK2 in a tumor-like environment, the anti-DKK2 antibody blocks tumor growth.

In a study using mice, anti DKK2 significantly inhibited tumor development in the KrasG12D/+ APCfl/fl model. Mice were infected with the adeno-Cre virus at age 10 weeks and divided into two groups. At twelve weeks, mice were randomly treated with the anti-DKK2 antibody 5F8 or with control anti-DKK2 IgG at 10 mg/kg once per week. Mice were sacrificed at 16 weeks and tumors were stained with methylene blue. After staining tumors with methylene blue was completed, they were measured using a stereomicroscope. The percentage of tumors was determined.

The DKK2 marker is thought to play a role during colorectal cancer metastasis. Research has shown that expression of DKK2 in the original tumor correlates with metastasis. DKK2 plays a key role in tumor cell proliferation and metastasis. If DKK2 is targeted, it may be possible reduce the growth and retard the progression of colorectal disease.

Recent research has shown that anti-DKK2 can block the VEGF/A/VEGFR pathway. This pathway in turn inhibits the maturation process of dendritic and MDSC cells, as well as activating CD8+ T cell. Anti-DKK2 increased IFNg in CD8+T cell and NK NK cells by blocking VEGFR.

Another treatment option is to combine anti-VEGFR with DKK2 blocking. Combining antiVEGFR and anti DKK2 blockade has provided significant long-term survival benefits. These results are encouraging and show the potential therapeutic value of anti-DKK2 antigen. This therapy could have a profound impact on the development of cancer immunotherapy.

Patients with SLE had higher serum and urine levels of DKK-1 than patients who were not LN-SLE controls. Combining serum DKK-1 with anti–dsDNA antibodies improved specificity. However, the accuracy of this diagnostic test was not significantly improved by using either anti–DKK2 antibody or anti–FZD-8. This antibody can be used to detect early stages of disease in patients suffering from active LN.

Boster Bio Anti FZD-8 antibody

The Boster bio Anti-FZD-8 antibody is used in IHC/P assays. The Boster Bio Anti-FZD-8 antibody uses the DKK2 marker and reacts with Human and Mouse cells. The antibody can also come as a blocker peptide that can be purchased separately. This product can be used to study fibroblasts.