EME1 Antibodies

AND EME1 ELISA kits, EME1 Proteins

Many biological assays use antibodies to detect EME1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with EME1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop EME1 antibodies...

We validate the specificity of these antibodies to EME1 by testing them on tissues known to express EME1 positively and negatively. Browse below to find the EME1 antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for EME1? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the EME1 IHC Guide

EME1 Infographic by Boster Bio

All EME1 Products

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Specificity

Applications

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Anti-EME1 Antibody Picoband®

IHC analysis of EME1 using anti-EME1 antibody (PB9804)

Flow Cytometry analysis of U20S cells using anti-EME1 antibody (PB9804)

Human, Rat

Flow Cytometry, IHC, IHC-F, ICC, WB

$370
Cat # PB9804
100 μg/vial

EME1 (NM_001166131) Human Over-expression Lysate

Human

$628
Cat # LS146956
20 µg, 100 µg

EME1 Overview

Facts about Crossover junction endonuclease EME1.

EME1 3D structure. Source: alphafold

Crossover junction endonuclease EME1 (EME1)

Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end in the branch nick. Typical substrates include 3'- flap structures, replication forks and nicked Holliday junctions.

May be required in mitosis for the processing of stalled or collapsed replication forks. .

Gene Information

Human
Gene Name:	EME1
Uniprot:	Q96AY2
Entrez:	146956

Family

Belongs to:

EME1/MMS4 family

Alternative Names

crossover junction endonuclease EME1; EC 3.1.22; EC 3.1.22.-; essential meiotic endonuclease 1 homolog 1 (S. pombe); essential meiotic endonuclease 1 homolog 2; FLJ31364; hMMS4; homolog of yeast EME1 endonuclease; MMS4 homolog; MMS4; MMS4L; SLX2 structure-specific endonuclease subunit homolog A; SLX2A

Molecular Weight

Mass (kDA):
63.252 kDA

Genomic Context


Human

Location:	17q21.33
Sequence:	17; NC_000017.11 (50373220..50381483)

Subcellular Localizations

Nucleus, nucleolus. Recruited to regions of DNA damage in S-phase cells.

Diseases

Malignant Neoplasms
Genomic Instability
Neoplasms
Cell Transformation, Neoplastic
Malignant Tumor Of Colon
Haploinsufficiency
Cell Invasion
Fanconi Anemia
Glioblastoma Multiforme
Hereditary Diseases
Congenital Abnormality
Anemia
Glioma

Glioblastoma
Polyploidy
Brain Neoplasms
Pancytopenia
Immunologic Deficiency Syndromes
Bloom Syndrome
Tumor Escape

Interacting Proteins

MUS81
SLX4

Pathways

Dna Replication
Dna Repair
Meiosis
S Phase
Hypersensitivity
Gene Conversion
Cell Cycle
Localization
Mismatch Repair
Transport
Cell Cycle Checkpoint
Dna Recombination
Chromosome Segregation

V(d)j Recombination
Double-strand Break Repair
Strand Invasion
Translation
Dna Damage Checkpoint
Chromatin Modification
Response To Hydroxyurea

PTMs

Cleavage

Phosphorylation

Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/EME1

References

PMID: 12686547 by Oegruenc M., et al. Identification and characterization of human MUS81-MMS4 structure- specific endonuclease.

PMID: 12721304 by Ciccia A., et al. Identification and characterization of the human mus81-eme1 endonuclease.