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Kelch-like protein 12 Antibodies
AND KLHL12 ELISA kits, Kelch-like protein 12 Proteins
Many biological assays use antibodies to detect Kelch-like protein 12, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Kelch-like protein 12 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Kelch-like protein 12 antibodies...
Designing a Western blot for Kelch-like protein 12? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the KLHL12 Western Blot GuideKLHL12 Infographic by Boster Bio
All KLHL12 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A08568-2
100 μg/vial
Cat # A08568-2
100 μg/vial
Human, Mouse, Rat
IHC, WB
$370
Cat # M08568-1
100 μg/vial
Cat # M08568-1
100 μg/vial
Human, Mouse, Rat
IHC, WB
$523
Cat # M08568
100 µl
Cat # M08568
100 µl
KLHL12 Overview
Facts about Kelch-like protein 12.
Kelch-like protein 12 (KLHL12)
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport (PubMed:22358839, PubMed:27565346). The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a crucial role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) (PubMed:22358839, PubMed:27565346).
The BCR(KLHL12) complex is also involved in neural crest specification: in response to cytosolic calcium increase, interacts with the heterodimer formed with PEF1 and PDCD6/ALG-2, resulting in bridge together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), boosting monoubiquitination of SEC31 and subsequent collagen export (PubMed:27716508). Included in the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3 (PubMed:16547521).
Gene Information
| Human | |
|---|---|
| Gene Name: | KLHL12 |
| Uniprot: | Q53G59 |
| Entrez: | 59349 |
Family
| Belongs to: |
|---|
| No superfamily |
Alternative Names
C3IP1kelch-like protein 12; CUL3-interacting protein 1; DKIR; FLJ27152; kelch-like 12 (Drosophila); kelch-like protein C3IP1
Molecular Weight
Mass (kDA):
63.277 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 1q32.1 |
| Sequence: | 1; NC_000001.11 (202891116..202928628, complement) |
Tissue Specificity
Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland.
Subcellular Localizations
Cytoplasmic vesicle, COPII-coated vesicle.
Diseases
- Arthritis
- Dysplasia
- Malnutrition
- Sjogren's Syndrome
- Salivary Gland Diseases
- Neoplasms
- Lupus Erythematosus, Systemic
- Attention Deficit Hyperactivity Disorder
- Chylomicron Retention Disease
Interacting Proteins
- ATXN1
- BAG6
- C1QTNF2
- CCBE1
- COL8A1
- CUL3
- DVL3
- FGD2
- GLYCTK
- INMT
Pathways
- Localization
- Transport
- Oocyte Development
- Secretion
PTMs
Research Areas
- DNA Repair
- DNA replication, Transcription, Translation and Splicing
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/KLHL12
bosterbio.com/bosterbio-gene-info-cards/KLHL12
References
PMID: 15383316 by Mai A., et al. hDKIR, a human homologue of the Drosophila kelch protein, involved in a ring-like structure.
PMID: 16108817 by Uchida K., et al. Identification of specific autoantigens in Sjoegren's syndrome by SEREX.