PHEX Antibodies

Phosphate-regulating neutral endopeptidase PHEX (PHEX)

Peptidase that cleaves SIBLING (small integrin-binding ligand, N-linked glycoprotein)-based ASARM peptides, thus regulating their biological activity (PubMed:9593714, PubMed:15664000, PubMed:18162525, PubMed:18597632). Cleaves ASARM peptides between Ser and Glu or Asp residues (PubMed:18597632).

Regulates osteogenic cell differentiation and bone mineralization through the cleavage of this MEPE-derived ASARM peptide (PubMed:18597632). Promotes dentin mineralization and renal phosphate reabsorption by cleaving DMP1- and MEPE-derived ASARM peptides (PubMed:18597632, PubMed:18162525).

Gene Information

Human
Gene Name:	PHEX
Uniprot:	P78562
Entrez:	5251

Family

Belongs to:
peptidase M13 family

Alternative Names

Phosphate-regulating neutral endopeptidase PHEX

Molecular Weight

Mass (kDA):
86.474 kDA

Genomic Context

Human
Location:	Xp22.11
Sequence:	X; NC_000023.11 (22032325..22251310)

Tissue Specificity

Specifically expressed in ovary (PubMed:9070861). Expressed at low levels in kidney (PubMed:9070861).

Subcellular Localizations

Cell membrane; Single-pass type II membrane protein.

Diseases

• Hypophosphatemic rickets, X-linked dominant (XLHR)

PTMs

• Disulfide bond

• Glycoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PHEX

Boster Bio - Best Uses Of The PHEX Marker

If you are an immunohistologist, you've probably heard of Boster Bio, a company that has been in business since 1993. Their signature products include antibodies and ELISA kits. Recently, they have added PCR-related molecular biology products. Their diverse line of products and services caters to a variety of scientists, including biochemists, biologists, and cell and molecular biologists. They provide technical support 24 hours a day and free resources for scientists.

Boster Bio optimizes antibodies for IHC

ELISA kits and research antibodies developed by Boster Bio detect biomarkers in cancer, neurosciences, developmental biology, and inflammation. These antibodies reach a picogram level of sensitivity. Boster Bio offers picogram level sensitivity ELISA kits and immunological reagents through tebu-bio. Boster Bio antibodies have been tested against a panel of 250 human tissues and untransfected cell lines to ensure optimum performance in your experiments.

The PHEX marker was developed by Steven Boster in 1993. His work led to the creation of a new product called a PHEX-compatible ELISA and hundreds of primary antibodies. He eventually became the largest antibody catalog in China by the late 90s. He also developed a proprietary ELISA platform called PicoKine(tm), which uses his trade secrets to deliver high-sensitivity ELISA kits.

IHC is a laboratory diagnostic technique based on the specific binding of an antibody to an antigen in tissue. Using a light microscope to visualize the binding of antibodies to antigens, it reveals the localization of cell components and their distribution in tissue. IHC is a highly versatile tool in both clinical and research settings. The use of antibodies to detect specific antigens is becoming an indispensable ancillary technique in anatomic pathology. Several new methods have become available that make it easy to analyze high volumes of samples.

Boster optimizes antibodies for WB

One of the most important aspects of WB immunoassays is the proper sample preparation. Unless the sample is prepared specifically for a specific antibody, the antibodies may not perform as well as they could. However, there are a number of sample preparation guides and protocols that can greatly improve the quality of your results. Among these guides and protocols are those from Boster Bio.

Boster optimizes antibodies for IHC

To help researchers improve their results, Boster Bio offers isotype controls, secondary antibodies, and detection systems optimized for IHC. In this article, we answer some common questions about negative control conditions and the IHC assays themselves. Read on to learn more about the advantages of IHC. Boster Bio is one of the leading suppliers of secondary antibodies, isotype controls, and detection systems for immunohistochemistry (IHC).

Immunohistochemistry (IHC) is a common ancillary test in anatomic pathology. It utilizes antibodies against specific antigens to determine cell type, origin, and phenotype. While most immunohistochemical tests are performed on formalin-fixed paraffin-embedded tissue, there are several methods for imaging antigens on tissue. For optimal results, consider the specimen type and the desired antigen.

To minimize the risk of false negative results, make sure the tissue is dried properly and that no air bubbles remain in the reagents. Also, make sure the tissue is blocked prior to staining. This step minimizes the potential for false positives due to nonspecific protein binding. If you choose an incorrect concentration, or use a longer incubation time, you may end up with a false negative.

A biopanned library of rabbit monoclonal antibodies is one of the most effective methods for identifying high-affinity, target-specific antibodies. It is an excellent resource for academic research and antibody-based therapeutics. The library is available in scFv format. A phage-display selection process is an alternative method to hybridoma technology.