Ras-related protein Ral-A Antibodies

Ras-related protein Ral-A (RALA)

Multifunctional GTPase involved in many different cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with different downstream effectors.

Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling (PubMed:20005108).

Gene Information

Human
Gene Name:	RALA
Uniprot:	P11233
Entrez:	5898

Family

Belongs to:
small GTPase superfamily

Alternative Names

RalA; Rasl1

Molecular Weight

Mass (kDA):
23.567 kDA

Genomic Context

Human
Location:	7p14.1
Sequence:	7; NC_000007.14 (39622955..39708124)

Subcellular Localizations

Cell membrane; Lipid-anchor; Cytoplasmic side. Cleavage furrow. Midbody, Midbody ring. Predominantly at the cell surface in the absence of LPA. In the presence of LPA, colocalizes with LPAR1 and LPAR2 in endocytic vesicles (PubMed:19306925). May colocalize with CNTRL/centriolin at the midbody ring (PubMed:16213214). However, localization at the midbody at late cytokinesis was not confirmed (PubMed:18756269).

Diseases

• Neoplasms
• Malignant Neoplasms
• Cell Transformation, Neoplastic
• Neoplasm Metastasis
• Carcinoma
• Malignant Paraganglionic Neoplasm
• Cell Invasion
• Tissue Adhesions
• Malignant Neoplasm Of Urinary Bladder
• Neoplasm Invasiveness
• Bladder Neoplasm
• Pancreatic Neoplasm

• Adenocarcinoma
• Malignant Neoplasm Of Pancreas
• Malignant Neoplasm Of Prostate
• Metaplastic Cell Transformation
• Prostatic Neoplasms
• Lung Neoplasms
• Leukemia

Interacting Proteins

• Exoc2
• PPP2R1B
• RALBP1

Pathways

• Exocytosis
• Localization
• Secretion
• Cell Migration
• Cell Death
• Cell Growth
• Transport
• Endocytosis
• Cell Proliferation
• Cell Cycle
• Rna Interference
• Cell Motility
• Oncogenesis

• Cell Adhesion
• Cytokinesis
• Wound Healing
• Mitosis
• Membrane Fusion
• Prenylation
• Mitochondrial Fission

PTMs

• Phosphorylation
• Ribosylation
• Cleavage
• Farnesylation
• Geranylgeranylation
• Prenylation

• Dephosphorylation
• Methylation
• Glycosylation
• Acylation
• Ubiquitination
• Myristoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RALA

Ana Boster Bio: Best Uses Of The RALA Marker Review

This Ana Boster Bio: Best Uses Of TheRALA Marker review will explore the history of Steven Boster, the RALA marker and its various uses. We'll also discuss how the high-affinity primary antibodies have been developed and used. In addition, we'll take a look at what ELISA and Western Blotting mean for this antibody. High-affinity primary antibodies are the most important component of RALA-based immunoassays.

Ana Boster Bio: Best Uses Of The RALA Marker

Whether you are a bioinformatician or a physician, you are faced with many choices when conducting an ELISA experiment. From the sample preparation process to the blocking buffer, the choices are numerous. To make your experiment run as smoothly as possible, read the tips and guides offered by Ana Boster Bio. She will explain the best practices for each step in the ELISA process.

Steven Boster's history

Steve Boster's life span spans over 40 years, but his legacy will endure far longer. Born in Joliet, IL, Steve was a career retail manager for decades. He also served in the U.S. Army. In addition to his family, he was a member of Concordia Hall in Staunton, VA. He leaves behind two daughters, Natosha Peck and Crystal Boster, and six grandchildren. In addition to his family, Steve left behind 4 Brothers and two sisters, Sandra Blanton and Jack Boster. He will be missed by his nieces and nephews.

As a young man, Steven Boster's first products were geared towards IHC. Over time, his company developed hundreds of primary antibodies and became the largest antibody catalog company in China. By the late 90s, Steven Boster had developed the PicoKine(tm) ELISA platform, which uses proprietary trade secrets to produce high-sensitivity ELISA kits. Steven Boster's history is littered with other impressive achievements.

High-affinity primary antibodies

Primary antibodies recognize a unique epitope with high affinity and specificity. Primary antibodies are available as polyclonal or monoclonal antibodies and are used in immunoassays for detecting specific biomolecules or measuring changes in these molecules. At GenScript, we offer over 1000 high-affinity primary antibodies with proven performance in several applications. Whether you need monoclonal antibodies or polyclonal antibodies for your research, GenScript has a wide selection to meet your needs.

FITC-SP1 is a monoclonal antibody that is capable of entering mammalian cells. This antibody has high affinity for the RalB receptor, and it inhibits the interaction between RalB and Sec5. It is also capable of penetrating the cell membrane, resulting in disruption of RalB-Sec5 interactions within the cell. A Western blot will visualize the relative intensity of the Sec5 band when compared to a no-peptide control.

The affinity of the antibodies is determined by measuring the KD value, or the rate of association and dissociation. The KD value is inversely proportional to the concentration of the two reactants, and the lower the KD value, the higher the affinity. The data were processed and reviewed by scientists at UC Davis. In the case of RabMAb antibodies, the KD value is 7 x 10-11 M, a measurement that indicates the high affinity of the antibody.

A mouse anti-RalB antibody was used as a positive control. This antibody was a kind gift from Larry Feig at Tufts University. The antibodies were separated by SDS-PAGE and quantified using an ECL chemiluminescence detection system. A pull-down assay was also performed, using the GST-RalGDS-RBD. The pull-down assay used this marker to determine the antigen-antibody complexes.

The RalB binding affinity can be increased by stapling the peptides. However, it is important to note that RalB binding affinity is extremely low, and that it is unlikely to be therapeutically useful in humans. This is because FITC-SP1 is highly competitive with Ral effectors and therefore weak. In addition, the SP1 and peptide 1 binding affinity are too low for reliable fitting.

Several other factors that affect the quality of the staining are important, including the concentration of primary antibodies, diluent, incubation time, and temperature. The optimal concentration of antibodies is one that is consistent across all parameters. A constant temperature and time of incubation is recommended to achieve a consistent signal. However, it is important to note that the two methods of analysis can be incompatible with each other.

A single B cell clone produces multiple monoclonal antibodies with specificity to a single epitope. Polyclonal antibodies, on the other hand, bind multiple epitopes on the same protein. Monoclonal antibodies are the best choice for studies that require a single member of a protein family. However, the RALA marker is more expensive than monoclonal antibodies.