TIGAR Antibodies

AND TIGAR ELISA kits, TIGAR Proteins

Many biological assays use antibodies to detect TIGAR, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with TIGAR in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop TIGAR antibodies...

We validate the specificity of these antibodies to TIGAR by testing them on tissues known to express TIGAR positively and negatively. Browse below to find the TIGAR antibody that suites your experiment. We have 2 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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TIGAR Infographic by Boster Bio

All TIGAR Products

Product

Figures

Specificity

Applications

Price

TIGAR (NM_020375) Human Recombinant Protein

$1249
Cat # PROTQ9NQ88
20 µg

TIGAR (NM_020375) Human Over-expression Lysate

Human

$628
Cat # LS057103
100 µg

TIGAR Overview

Facts about Fructose-2,6-bisphosphatase TIGAR.

TIGAR 3D structure. Source: alphafold

Fructose-2,6-bisphosphatase TIGAR (TIGAR)

Fructose-bisphosphatase hydrolyzing fructose-2,6- bisphosphate as well as fructose-1,6-bisphosphate (PubMed:19015259). Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent fashion, resulting in the pentose phosphate pathway (PPP) activation and NADPH production (PubMed:16839880, PubMed:22887998).

Contributes to the creation of reduced glutathione to create a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death (PubMed:16839880, PubMed:19713938, PubMed:23726973, PubMed:22887998, PubMed:23817040). Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase action (PubMed:23185017).

Gene Information

Human
Gene Name:	TIGAR
Uniprot:	Q9NQ88
Entrez:	57103

Family

Belongs to:

phosphoglycerate mutase family

Alternative Names

C12orf5; chromosome 12 open reading frame 5; EC 3.1.3.46; probable fructose-2,6-bisphosphatase TIGAR; TIGAR; TIGARTP53-induced glycolysis and apoptosis regulator; transactivated by NS3TP2 protein

Molecular Weight

Mass (kDA):
30.063 kDA

Genomic Context


Human

Location:	12p13.32
Sequence:	12; NC_000012.12 (4321213..4360028)

Tissue Specificity

Expressed in the brain (PubMed:22887998). Expressed in breast tumors (PubMed:21820150). Expressed in glioblastomas (PubMed:22887998).

Subcellular Localizations

Cytoplasm. Nucleus. Mitochondrion. Translocated to the mitochondria during hypoxia in a HIF1A-dependent manner (PubMed:23185017). Colocalizes with HK2 in the mitochondria during hypoxia (PubMed:23185017). Translocated to the nucleus during hypoxia and/or genome stress-induced DNA damage responses in cancer cells (PubMed:25928429). Translocation to the mitochondria is enhanced in ischemic cortex after reperfusion and/or during oxygen and glucose deprivation (OGD)/reoxygenation insult in primary neurons (By similarity).