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Product Info Summary
| SKU: | EK0945-BV |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Bovine |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Bovine Endothelin ELISA Kit PicoKine®
SKU/Catalog Number
EK0945-BV
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Bovine Endothelin ELISA Kit PicoKine™ (96 Tests). Quantitate Bovine Endothelin in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Bovine Endothelin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0945-BV)
Clonality of Antibodies
See Datasheet for details
Immunogen
A peptide reflecting the target protein in bovine. Sequence: C53-W73
Sensitivity
<0.5 pg/ml
Assay Range
3.9 pg/ml - 250 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0945-BV for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0945-BV is reactive to in Bovine samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK0945-BV is guaranteed for ELISA in Bovine by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of
The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of Bovine endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0945-BV-CAP | Anti-Bovine Pre-coated 96-well strip microplate | 1 |
| EK0945-BV-ST | Bovine Standard | 2 vials, 1 ng/tube |
| EK0945-BV-DA | Bovine Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 3.9 | 7.8 | 15.6 | 31.2 | 62.5 | 125 | 250 |
| O.D. | 0.017 | 0.045 | 0.088 | 0.157 | 0.357 | 0.772 | 1.646 | 2.039 |
Data Example Images
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Bovine Endothelin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Bovine Endothelin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 26.1 | 85.4 | 146 | 23.2 | 84.3 | 143 |
| Standard deviation | 1.41 | 3.76 | 8.03 | 1.79 | 6.07 | 8.72 |
| CV (%) | 4.4% | 4.4% | 5.5% | 7.7% | 7.2% | 6.1% |
Specific Publications For Bovine Endothelin ELISA Kit PicoKine® (EK0945-BV)
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Customer Q&As
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16 Customer Q&As for Bovine Endothelin ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for Endothelin-1 ELISA after freezing?
J. Garcia
Verified customer
Asked: 2020-04-29
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Endothelin-1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-04-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-03-31
Question
How much concentration of sodium azide or thimerosal and 2-mercaptoethanol (or other toxic substances) is contained in EK0945-BV?
Verified customer
Asked: 2019-08-28
Answer
For the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV), Recombinant standard - sodium azide 0.02mg/vial Biotinylated antibody, sample, antibody, and abc diluent buffer - sodium azide 0.02%
Boster Scientific Support
Answered: 2019-08-29
Question
Does EK0945-BV contain protein derived from Baculovirus expression system?
Verified customer
Asked: 2019-08-28
Answer
For the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV), the expression system for standard is Bovine peptide.
Boster Scientific Support
Answered: 2019-08-29
Question
Does your shipping medium include animal origin components?
Verified customer
Asked: 2019-08-28
Answer
The shipping medium does not include animal origin components.
Boster Scientific Support
Answered: 2019-08-29
Question
What is the composition of the reaction stop solution for EK0945-BV?
Verified customer
Asked: 2019-08-28
Answer
The TMB stop solution for the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV) is Sulphuric acid - 2N.
Boster Scientific Support
Answered: 2019-08-29
Question
What does the abc diluent buffer for EK0945-BV contain?
Verified customer
Asked: 2019-08-27
Answer
ABC duiluent buffer for the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV) contains 0.02% sodium azide, 0.5% BSA, 0.2% Tris and 1% Nacl.
Boster Scientific Support
Answered: 2019-08-29
Question
Is the expression system for standard of EK0945-BV an unpurified peptide derived from cattle?
Verified customer
Asked: 2019-08-27
Answer
The immunogen of the standard for the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV) is synthesized peptide.
Boster Scientific Support
Answered: 2019-08-29
Question
Q: how to analyze ELISA data? I measured Endothelin-1 level in plasma.
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-05-15
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-01-11
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-01-11
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Endothelin-1 using the EK0945-BV Bovine Endothelin-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-11-18
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-11-18
Question
# A reagent containing sodium azide, but please let me know exactly what abc diluent buffer is. # Expression system for standard: Bovine peptide. Please let me know if this is an unpurified peptide derived from cattle. # stop solution: Sulfuric acid-2N The MSDS stated 2%. Please let me know which is correct, as the concentration is completely different between 2% and 2N.
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
Please see the following answeres. # A reagent containing sodium azide, but please let me know exactly what abc diluent buffer is. ABC duiluent buffer contains 0.02% sodium azide, 0.5% BSA, 0.2% Tris and 1% Nacl. # Expression system for standard: Bovine peptide. Please let me know if this is an unpurified peptide derived from cattle. The immunogen of the standard is synthesized peptide. # stop solution: Sulfuric acid-2N The MSDS stated 2%. Please let me know which is correct, as the concentration is completely different between 2% and 2N. The stop solution is 2% sulfuric acid.
Boster Scientific Support
Answered: 2018-05-22
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-05-17
Question
Q: is it okay to use citrate plasma as samples in Bovine Endothelin-1 Picokine® ELISA Kit (Catalog # EK0945-BV)?
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Endothelin-1 causing degradation of its protein structure. Endothelin-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Endothelin-1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-03-16
Question
Q: What is the optimal O.D. value for Endothelin-1 ELISA kit? I performed your Endothelin-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Endothelin-1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Endothelin-1 ELISA kit to have sensitivity of 0.5pg/ml, that means the minimum amount of Endothelin-1 that can be declared/interpreted as positive by the above standard is 0.5pg/ml.
Boster Scientific Support
Answered: 2017-09-21
Question
Q: if the enzyme conjugated Endothelin-1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Endothelin-1 antigen?
K. Martin
Verified customer
Asked: 2016-05-15
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-05-15
