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Product Info Summary
| SKU: | PROTP12758 |
|---|---|
| Size: | Starting from 20ug |
| Source: | E. coli |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
e.coli uDP Recombinant Protein
SKU/Catalog Number
PROTP12758
Size
Starting from 20ug
Tag
His-Tag
Description
E. coli uDP Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-253aa. Application(s): Enzyme Activity, SDS-PAGE.
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
Cite This Product
e.coli uDP Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP12758)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50mM NaCl
Concentration
1mg/ml (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Predicted MW
29.3 kDa (273aa) confirmed by MALDI-TOF
Amino Acid Sequence
E.coli, P12758, 1-253aa; MGSSHHHHHH SSGLVPRGSH MSKSDVFHLG LTKNDLQGAT LAIVPGDPDR VEKIAALMDK PVKLASHREF TTWRAELDGK PVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP HINVGDVLVT TASVRLDGAS LHFAPLEFPA VADFECTTAL VEAAKSIGAT THVGVTASSD TFYPGQERYD TYSGRVVRHF KGSMEEWQAM GVMNYEMESA TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH AVKIVVEAAR RLLBioactivity
Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of uridine presence of phosphate per minute at pH 7.5 at 25°C.Innovating Scientists Reward
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Assay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Specific Publications For e.coli uDP Recombinant Protein (PROTP12758)
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