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Product Info Summary
| SKU: | BA1120 |
|---|---|
| Size: | 0.5ml |
| Application: | Flow Cytometry, IF |
Product info
| Product Name | FITC Conjugated Protein A |
| SKU/Catalog Number | BA1120 |
| Pack Size | 0.5ml |
| Form | Concentrated, Liquid |
| Concentration | 1 mg/mL |
| Description | Protein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR. It has found use in biochemical research because of its ability to bind immunoglobulins. It is composed of five homologous Ig-binding domains that fold into a three-helix bundle. Each domain is able to bind proteins from many mammalian species, most notably IgGs (Table 1). It binds the heavy chain within the Fc region of most immunoglobulins and also within the Fab region in the case of the human VH3 family. Through these interactions in serum, where IgG molecules are bound in the wrong orientation (in relation to normal antibody function), the bacteria disrupts opsonization and phagocytosis. Recombinant Protein A is produced in E.coli and essentially functions the same as native Protein A. Protein A may be conjugated with various reporter molecules, including fluorescent dyes (FITC), enzyme markers (peroxidase, b-galactosidase, and alkaline phosphatase), biotin, and colloidal gold without affecting the antibody binding site on the molecule. These conjugates are used to detect immunoglobulins in various immunochemical assays including Western blotting, immunohistochemistry, and ELISA applications. |
| Contents | 0.5 mg of FITC conjugated protein A, F/P=3/1; 0.01M PBS; 50% glycerol. |
| Labeling Method | Protein A is conjugated to FITC by means of a method described by Hijmans,W.,et al. (Reference: Hijmans,W.,et al. Clin.Exp.Immunol.,4, 457 (1969)) |
| Storage & Expiration | At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light. |
| Shipping | Ships in room temperature. Can also ship with gel ice or dry ice but not necessary. |
| Applications | IF, Flow Cytometry |
| Cite This Product | FITC Conjugated Protein A (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1120) |
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Validation Images & Assay Conditions
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PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti-PCK Antigen Affinity purified monoclonal antibody (Catalog # MA1081) at 1 μg/mL. FITC Conjugated Protein A (Catalog # BA1120) was used to detect the primary antibody at 20μg/mL.
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