HSF1 (Phospho-Ser121) Colorimetric Cell-Based ELISA Kit

HSF1 ELISA kit, phospho-specific, cell-based

The HSF1 (Phospho-Ser121) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSF1 (Phospho-Ser121) protein expression profile in cells. The kit can be used for measuring the relative amounts of HSF1 (Phospho-Ser121) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSF1 (Phospho-Ser121).

Product Info Summary

SKU: EKC2552
Size: 1 kit, containing two 96-well plates and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA

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Product Name

HSF1 (Phospho-Ser121) Colorimetric Cell-Based ELISA Kit

See all HSF1 products

SKU/Catalog Number

EKC2552

Size

1 kit, containing two 96-well plates and all necessary reagents

Description

The HSF1 (Phospho-Ser121) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSF1 (Phospho-Ser121) protein expression profile in cells. The kit can be used for measuring the relative amounts of HSF1 (Phospho-Ser121) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSF1 (Phospho-Ser121).

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

HSF1 (Phospho-Ser121) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2552)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC2552 is reactive to HSF1 in Human, Mouse samples

Application Guarantee

EKC2552 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 2 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-HSF1 (Phospho-specific) Antibody (Rabbit Polyclonal) 60 µl (100x) Red
100x Anti-HSF1 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 12ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 12ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 12ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For HSF1 (Source: Uniprot.Org, NCBI)

Gene Name

HSF1

Full Name

Heat shock factor protein 1

Weight

57.26kDa

Superfamily

HSF family

Alternative Names

heat shock factor protein 1; heat shock transcription factor 1HSTF1HSF 1; HSF1; HSTF 1; HSTF1 HSF1 HSTF1 heat shock transcription factor 1 heat shock factor protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HSF1, check out the HSF1 Infographic

HSF1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HSF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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