Product Info Summary
SKU: | EK7102 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Serum and Plasma |
Product info
Product Name
Human 25(OH) Vitamin D ELISA Kit (Competitive EIA)
SKU/Catalog Number
EK7102
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Human 25 (OH) Vitamin D ELISA Kit, tested with Serum and Plasma. Format: 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human 25(OH) Vitamin D ELISA Kit (Competitive EIA) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7102)
Clonality of Antibodies
See Datasheet for details
Sensitivity
2.5 ng/ml
Assay Range
2.5-150 ng/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7102 is reactive to in Human samples
Validated Sample Types
Serum and Plasma
Application Guarantee
EK7102 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity |
1. Microwell plate coated with anti-Vitamin D | 12x8x1 |
2. Vitamin D Standard Set: 7 vials (ready to use) | 0.5 ml |
3. Vitamin D Control Set: 2 vials (ready to use) | 0.5 ml |
4. Biotinylated 25 (OH)D Reagent: 1 Vial (51X) | 0.55 ml |
5. Assay Diluent, 1 bottle | 24 ml |
6. Streptavidin-HRP, 1 bottle (ready to use) | 23 mL |
7. Stop Solution, 1 bottle (ready to use) | 12 mL |
8. TMB Substrate, 2 bottles (ready to use) | 2 x 12 ml |
9. Microplate sealing film | 2 |
10. Wash Concentrate 20X, 1 bottle | 25 ml |
Standard Concentrations and example data
OD 450 nm | 25(OH)D, (ng/ml) | |
---|---|---|
Std 1 | 2.4 | 0 |
Std 2 | 2.1 | 2.5 |
Std 3 | 1.76 | 5 |
Std 4 | 1.31 | 15 |
Std 5 | 0.79 | 35 |
Std 6 | 0.48 | 70 |
Std 7 | 0.29 | 150 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster product! Applicable to all scientists worldwide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Data Example Images
Click image to see more details
Boster Kit Box
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, ELISA Target remove.
Protein Target Info & Infographic
Gene/Protein Information For (Source: Uniprot.org, NCBI)
Gene Name
Full Name
Weight
For more info on , check out the Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for : database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human 25(OH) Vitamin D ELISA Kit (Competitive EIA) (EK7102)
Hello CJ!
EK7102 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effectiveness of Sublingual Immunotherapy in the Treatment of HDM-Induced Nasobronchial Allergies: A 3-Year Randomized Case-Control Study From Kashmir
Improved pulmonary function test (PFT) after 1 One year of Sublingual Immunotherapy (SLIT) as adjunct to pharmacotherapy in Mild Allergic Asthmatics
Influence of single gene variants of FOXP3 on allergic asthma predisposition
Beigh AH,Rasool R,Kawoosa F,Manzoor S,Rashid R,Andrabi KI,Shah ZA,Qureshi T. Improved pulmonary function test (PFT) after 1 One year of Sublingual Immunotherapy (SLIT) as adjunct to pharmacotherapy in Mild Allergic Asthmatics. Immunol Lett. 2020 Dec 16:S0
Species: Human
EK7102 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human 25(OH) Vitamin D ELISA Kit (Competitive EIA)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Human 25(OH) Vitamin D ELISA Kit (Competitive EIA)
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question