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Product Info Summary
| SKU: | EK2246 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
Product info
Product Name
Human ASAHL ELISA Kit PicoKine®
View all ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase ELISA kits
SKU/Catalog Number
EK2246
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ASAHL PicoKine™ Quick ELISA Kit PicoKine ELISA Kit® (96 Tests). Quantitate Human ASAHL in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 8 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ASAHL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2246)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: M1-K359
Sensitivity
<8 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2246 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2246 is reactive to NAAA in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Application Guarantee
EK2246 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase
N-acylethanolamine-hydrolyzing acid amidase is an enzyme that in humans is encoded by the NAAA gene. Enables N-(long-chain-acyl)ethanolamine deacylase activity; N-acylsphingosine amidohydrolase activity; and fatty acid amide hydrolase activity. Involved in several processes, including N-acylethanolamine metabolic process; N-acylphosphatidylethanolamine metabolic process; and sphingosine metabolic process. Located in lysosome. Is extrinsic component of membrane.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2246-CAP | Anti-Human NAAA Pre-coated 96-well strip microplate | 1 |
| EK2246-ST | Human NAAA Standard | 2 vials, 10 ng/tube |
| EK2246-DA | Human NAAA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.026 | 0.048 | 0.165 | 0.211 | 0.434 | 0.816 | 1.254 | 2.016 |
Data Example Images
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Human ASAHL PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ASAHL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 30 | 48 | 437 | 25 | 34 | 452 |
| Standard deviation | 0.88 | 2.37 | 31.81 | 0.84 | 1.17 | 12.75 |
| CV(%) | 2.9% | 4.9% | 7.3% | 3.3% | 3.5% | 2.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 30 | 30 | 24 | 25 | 27 | 1.71 | 6.3% |
| Sample 2 | 48 | 36 | 49 | 59 | 48 | 2.75 | 5.7% |
| Sample 3 | 437 | 401 | 423 | 452 | 428 | 22.18 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For NAAA (Source: Uniprot.Org, NCBI)
Gene Name
NAAA
Full Name
N-acylethanolamine-hydrolyzing acid amidase
Weight
40.066kDa
Superfamily
acid ceramidase family
Alternative Names
Acid ceramidase-like protein; ASAHL; ASAH-like protein; EC 3.5.1.-; NAAA; N-acylethanolamine acid amidase; Nacylethanolaminehydrolyzing Acid Amidase; N-acylethanolamine-hydrolyzing acid amidase; N-acylsphingosine amidohydrolase (acid ceramidase)-like; N-acylsphingosine amidohydrolase-like; PLT NAAA ASAHL, PLT N-acylethanolamine acid amidase N-acylethanolamine-hydrolyzing acid amidase|ASAH-like protein|acid ceramidase-like protein|acylsphingosine deacylase NAAA
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NAAA, check out the NAAA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NAAA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ASAHL ELISA Kit PicoKine® (EK2246)
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