Product Info Summary
SKU: | EK2135 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine |
Product info
Product Name
Human Attractin ELISA Kit PicoKine®
SKU/Catalog Number
EK2135
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Attractin ELISA Kit PicoKine™ (96 Tests). Quantitate Human ATRN in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 20 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Attractin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2135)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: CHO; Immunogen sequence: A84-Q1272
Sensitivity
<20 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2135 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2135 is reactive to ATRN in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine
Application Guarantee
EK2135 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Attractin
Attractin is a protein that in humans is encoded by the ATRN gene. This gene encodes both membrane-bound and secreted protein isoforms. A membrane-bound isoform exhibits sequence similarity with the mouse mahogany protein, a receptor involved in controlling obesity. A secreted isoform is involved in the initial immune cell clustering during inflammatory responses that may regulate the chemotactic activity of chemokines.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK2135-CAP | Anti-Human ATRN Pre-coated 96-well strip microplate | 1 |
EK2135-ST | Human ATRN Standard | 2 vials, 50 ng/tube |
EK2135-DA | Human ATRN Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.003 | 0.165 | 0.321 | 0.560 | 0.936 | 1.457 | 1.926 | 2.344 |
Data Example Images
Click image to see more details
Human Attractin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Attractin, Dilution ratio of 1:5000, concentration in serum and plasma is 27.8-71 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Attractin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1352 | 5864 | 26013 | 1363 | 6055 | 26542 |
Standard deviation | 85.18 | 363.57 | 1586.79 | 94.05 | 387.52 | 1353.64 |
CV(%) | 6.3% | 6.2% | 6.1% | 6.9 | 6.4% | 5.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Attractin in ELISA kits from four different production batches/lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 1352 | 1388 | 1425 | 1501 | 1417 | 72.24 | 5.1% |
Sample 2 | 5864 | 5941 | 5263 | 5554 | 5656 | 214.91 | 3.8% |
Sample 3 | 26013 | 27512 | 26944 | 25414 | 26471 | 1191.18 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For ATRN (Source: Uniprot.Org, NCBI)
Gene Name
ATRN
Full Name
Attractin
Weight
158.537kDa
Alternative Names
ATRN; Attractin; DPPT-L; KIAA0548; Mahogany Protein; MGC126754; MGCA ATRN DPPT-L, MGCA attractin attractin|attractin-2|mahogany homolog|mahogany protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ATRN, check out the ATRN Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ATRN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Attractin ELISA Kit PicoKine® (EK2135)
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