Human Calsyntenin 1 ELISA Kit PicoKine®

Calsyntenin-1 ELISA kit for Human

Human Calsyntenin 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CLSTN1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EK2192
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human Calsyntenin 1 ELISA Kit PicoKine®

View all Calsyntenin-1 ELISA kits

SKU/Catalog Number

EK2192

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Calsyntenin 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CLSTN1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Calsyntenin 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2192)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: R30-T851

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2192 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2192 is reactive to CLSTN1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2192 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Calsyntenin-1

Calsyntenin-1 is a protein that in humans is encoded by the CLSTN1 gene. This gene is a member of the calsyntenin family, a subset of the cadherin superfamily. The encoded transmembrane protein, also known as alcadein-alpha, is thought to bind to kinesin-1 motors to mediate the axonal anterograde transport of certain types of vesicle. Amyloid precursor protein (APP) is trafficked via these vesicles and so this protein is being investigated to see how it might contribute to the mechanisms underlying Alzheimer's disease. Alternative splicing results in multiple transcript variants.

Kit Components

Catalog Number Description Quantity
EK2192-CAP Anti-Human CLSTN1 Pre-coated 96-well strip microplate 1
EK2192-ST Human CLSTN1 Standard 2 vials, 10 ng/tube
EK2192-DA Human CLSTN1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)015.631.262.51252505001,000
O.D.0.0160.0480.1350.2510.4440.8961.2742.056

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Calsyntenin 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2316345221174423
Standard deviation1.2711.2532.091.099.2225.8
CV(%)5.5%6.9%7.1%5.2%5.3%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Calsyntenin-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 123221920210.763.6%
Sample 21631651581561616.744.2%
Sample 345246345647046020.714.5%
*number of samples for each test n=16.

Gene/Protein Information For CLSTN1 (Source: Uniprot.Org, NCBI)

Gene Name

CLSTN1

Full Name

Calsyntenin-1

Weight

109.793kDa

Alternative Names

alcadein alpha 1; Alcadein-alpha; ALC-ALPHA; alcalpha1; alcalpha2; Alzheimer-related cadherin-like protein; cadherin-related family member 12; calsyntenin 1; Calsyntenin1; Calsyntenin-1; CDHR12; CLSTN1; CS1; CSTN1; FLJ32258; KIAA0911alcadein-alpha; Non-classical cadherin XB31alpha; non-classical cadherin XB31alpha1; PIK3CD; XB31alpha CLSTN1 ALC-ALPHA, CDHR12, CST-1, CSTN1, PIK3CD, XB31alpha, alcalpha1, alcalpha2 calsyntenin 1 calsyntenin-1|alcadein-alpha|alzheimer-related cadherin-like protein|cadherin-related family member 12|non-classical cadherin XB31alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CLSTN1, check out the CLSTN1 Infographic

CLSTN1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CLSTN1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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