Human Cathepsin C ELISA Kit PicoKine®

Product Name

Human Cathepsin C ELISA Kit PicoKine®

View all Cathepsin C/DPPI ELISA kits

SKU/Catalog Number

EK2177

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Cathepsin C ELISA Kit PicoKine™ (96 Tests). Quantitate Human CTSC in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Cathepsin C ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2177)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: D25-L463

Sensitivity

<75 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2177 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2177 is reactive to CTSC in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2177 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Cathepsin C/DPPI

Cathepsin C (CTSC) also known as dipeptidyl peptidase I (DPP-I) is a lysosomal exo-cysteine protease belonging to the peptidase C1 protein family, a subgroup of the cysteine cathepsins. In humans, it is encoded by the CTSC gene. This gene encodes a member of the peptidase C1 family and lysosomal cysteine proteinase that appears to be a central coordinator for activation of many serine proteinases in cells of the immune system. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate heavy and light chains that form a disulfide-linked dimer. A portion of the propeptide acts as an intramolecular chaperone for the folding and stabilization of the mature enzyme. This enzyme requires chloride ions for activity and can degrade glucagon. Defects in the encoded protein have been shown to be a cause of Papillon-Lefevre syndrome, an autosomal recessive disorder characterized by palmoplantar keratosis and periodontitis.

Kit Components

Catalog Number	Description	Quantity
EK2177-CAP	Anti-Human CTSC Pre-coated 96-well strip microplate	1
EK2177-ST	Human CTSC Standard	2 vials, 10 ng/tube
EK2177-DA	Human CTSC Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.007	0.015	0.054	0.105	0.226	0.587	1.156	1.879

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Cathepsin C/DPPI, Dilution ratio of 1:1, concentration in serum and plasma is 1616-2624 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Cathepsin C ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cathepsin C/DPPI in ELISA kits from four different production batches/lots.

Gene/Protein Information For CTSC (Source: Uniprot.Org, NCBI)

Alternative Names

Cathepsin C; cathepsin CEC 3.4.14.1; Cathepsin J; CPPIHMS; CTSC; dipeptidyl peptidase 1; Dipeptidyl peptidase I; Dipeptidyl transferase; dipeptidyl-peptidase I; DPP1; DPPI; DPP-I; JP; JPD; PALS; PLS CTSC CPPI, DPP-I, DPP1, DPPI, HMS, JP, JPD, PALS, PDON1, PLS cathepsin C dipeptidyl peptidase 1|cathepsin J|dipeptidyl transferase|dipeptidyl-peptidase I

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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