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Product Info Summary
| SKU: | EK2211 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CD42d ELISA Kit PicoKine®
View all Glycoprotein V/CD42d ELISA kits
SKU/Catalog Number
EK2211
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD42d Kit PicoKine™ (96 Tests). Quantitate Human GP5 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD42d ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2211)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q17-G560
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2211 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2211 is reactive to GP5 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2211 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Glycoprotein V/CD42d
Glycoprotein V (platelet) (GP5) also known as CD42d (Cluster of Differentiation 42d), is a human gene. Human platelet glycoprotein V (GP5) is a part of the Ib-V-IX system of surface glycoproteins that constitute the receptor for von Willebrand factor (VWF; MIM 613160) and mediate the adhesion of platelets to injured vascular surfaces in the arterial circulation, a critical initiating event in hemostasis. The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain (GP1BA; MIM 606672) and a beta chain (GP1BB; MIM 138720), that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with platelet glycoprotein IX (GP9; MIM 173515) and GP5. Mutations in GP1BA, GP1BB, and GP9 have been shown to cause Bernard-Soulier syndrome, a bleeding disorde.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2211-CAP | Anti-Human GP5 Pre-coated 96-well strip microplate | 1 |
| EK2211-ST | Human GP5 Standard | 2 vials, 10 ng/tube |
| EK2211-DA | Human GP5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.053 | 0.122 | 0.196 | 0.412 | 0.872 | 1.250 | 1.563 | 2.055 |
Data Example Images
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Human CD42d PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD42d ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 108 | 466 | 1635 | 118 | 474 | 1588 |
| Standard deviation | 5.51 | 20.97 | 78.48 | 4.6 | 19.43 | 76.22 |
| CV(%) | 5.1% | 4.5% | 4.8% | 3.9% | 4.1% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Glycoprotein V/CD42d in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 108 | 122 | 117 | 125 | 118 | 7.2 | 6.1% |
| Sample 2 | 466 | 462 | 501 | 481 | 478 | 26.26 | 5.5% |
| Sample 3 | 1635 | 1752 | 1693 | 1727 | 1702 | 100.4 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For GP5 (Source: Uniprot.Org, NCBI)
Gene Name
GP5
Full Name
Platelet glycoprotein V
Weight
60.959kDa
Alternative Names
platelet glycoprotein V; CD42d; glycoprotein V (platelet); Glycoprotein V; GP5; GPV GP5 CD42d, GPV glycoprotein V platelet platelet glycoprotein V|glycoprotein 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GP5, check out the GP5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GP5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD42d ELISA Kit PicoKine® (EK2211)
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