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Product Info Summary
| SKU: | EK2150 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CD7 ELISA Kit PicoKine®
SKU/Catalog Number
EK2150
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD7 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CD7 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD7 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2150)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: HEK293; Immunogen sequence: A26-P180
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2150 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2150 is reactive to CD7 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2150 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD7
CD7 (Cluster of Differentiation 7) is a protein that in humans is encoded by the CD7 gene. This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2150-CAP | Anti-Human CD7 Pre-coated 96-well strip microplate | 1 |
| EK2150-ST | Human CD7 Standard | 2 vials, 10 ng/tube |
| EK2150-DA | Human CD7 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 |
| O.D. | 0.015 | 0.058 | 0.125 | 0.221 | 0.474 | 0.860 | 1.254 | 2.256 |
Data Example Images
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Human CD7 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD7 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 22 | 165 | 451 | 26 | 177 | 426 |
| Standard deviation | 1.21 | 11.39 | 32.02 | 1.4 | 11.15 | 28.12 |
| CV(%) | 5.5% | 6.9% | 7.1% | 5.1% | 6.3% | 6.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD7 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 22 | 23 | 25 | 28 | 25 | 0.81 | 3.3% |
| Sample 2 | 165 | 157 | 164 | 163 | 162 | 7.79 | 4.8% |
| Sample 3 | 451 | 458 | 462 | 473 | 461 | 20.28 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For CD7 (Source: Uniprot.Org, NCBI)
Gene Name
CD7
Full Name
T-cell antigen CD7
Weight
25.409kDa
Alternative Names
CD7 antigen (p41); CD7 antigen; CD7 molecule; CD7; GP40T-cell surface antigen Leu-9; LEU-9; T-cell antigen CD7; T-cell leukemia antigen; Tp40; TP41; TP41p41 protein CD7 GP40, LEU-9, TP41, Tp40 CD7 molecule T-cell antigen CD7|CD7 antigen (p41)|T-cell leukemia antigen|T-cell surface antigen Leu-9|p41 protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD7, check out the CD7 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD7 ELISA Kit PicoKine® (EK2150)
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