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Product Info Summary
| SKU: | EK2157 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human CDO ELISA Kit PicoKine®
SKU/Catalog Number
EK2157
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CDO ELISA Kit PicoKine™ (96 Tests). Quantitate Human CDON in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CDO ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2157)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D26-P943
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2157 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2157 is reactive to CDON in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2157 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CDO
Cell adhesion molecule-related/down-regulated by oncogenes is a protein that in humans is encoded by the CDON gene. This gene encodes a cell surface receptor that is a member of the immunoglobulin superfamily. The encoded protein contains three fibronectin type III domains and five immunoglobulin-like C2-type domains. This protein is a member of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells and positively regulates myogenesis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2157-CAP | Anti-Human CDON Pre-coated 96-well strip microplate | 1 |
| EK2157-ST | Human CDON Standard | 2 vials, 10 ng/tube |
| EK2157-DA | Human CDON Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.035 | 0.072 | 0.121 | 0.220 | 0.398 | 0.755 | 1.347 | 2.005 |
Data Example Images
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Human CDO PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CDO, Dilution ratio of 1:1, concentration in serum and plasma is 1-2 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CDO ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 202 | 1756 | 4943 | 211 | 1963 | 4861 |
| Standard deviation | 12.52 | 133.46 | 370.73 | 14.56 | 113.85 | 286.8 |
| CV(%) | 6.2% | 7.6% | 7.5% | 6.9% | 5.8% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CDO in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 202 | 236 | 212 | 233 | 221 | 13.47 | 6.1% |
| Sample 2 | 1756 | 1788 | 1863 | 1923 | 1833 | 69.64 | 3.8% |
| Sample 3 | 4943 | 4465 | 4863 | 4812 | 4771 | 248.08 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For CDON (Source: Uniprot.Org, NCBI)
Gene Name
CDON
Full Name
Cell adhesion molecule-related/down-regulated by oncogenes
Weight
139.147kDa
Alternative Names
CDO; Cdon homolog (mouse); CDON; CDOORCAM; cell adhesion molecule-related/down-regulated by oncogenes; MGC111524; ORCAM; surface glycoprotein, Ig superfamily member CDON CDO1, HPE11, ORCAM, CDON cell adhesion associated, oncogene regulated cell adhesion molecule-related/down-regulated by oncogenes|Cdon homolog|surface glycoprotein, Ig superfamily member
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CDON, check out the CDON Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CDON: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CDO ELISA Kit PicoKine® (EK2157)
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