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Product Info Summary
| SKU: | EK2088 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human CRTAC1 ELISA Kit PicoKine®
SKU/Catalog Number
EK2088
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CRTAC1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CRTAC1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CRTAC1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2088)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S28-C661
Sensitivity
<50 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2088 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2088 is reactive to CRTAC1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2088 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CRTAC1
cartilage acidic protein 1 is a protein that in humans is encoded by the CRTAC1 gene. It is mapped to 10q24.2. This gene encodes a glycosylated extracellular matrix protein that is found in the interterritorial matrix of articular deep zone cartilage. This protein is used as a marker to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture. The presence of FG-GAP motifs and an RGD integrin-binding motif suggests that this protein may be involved in cell-cell or cell-matrix interactions. Copy number alterations in this gene have been observed in neurofibromatosis type 1-associated glomus tumors. Alternative splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2088-CAP | Anti-Human CRTAC1 Pre-coated 96-well strip microplate | 1 |
| EK2088-ST | Human CRTAC1 Standard | 2 vials, 20 ng/tube |
| EK2088-DA | Human CRTAC1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.006 | 0.037 | 0.071 | 0.140 | 0.253 | 0.483 | 0.922 | 1.810 |
Data Example Images
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Human CRTAC1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CRTAC1, Dilution ratio of 1:1, concentration in serum and plasma is around 7 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CRTAC1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 561 | 1709 | 6088 | 583 | 2016 | 7238 |
| Standard deviation | 23.56 | 73.49 | 249.61 | 38.48 | 145.15 | 550.09 |
| CV (%) | 4.2% | 4.3% | 4.1% | 6.6% | 7.2% | 7.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CRTAC1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 561 | 555 | 488 | 512 | 529 | 30.29 | 5.7% |
| Sample 2 | 1709 | 1635 | 1676 | 1433 | 1613 | 107.31 | 6.6% |
| Sample 3 | 6088 | 5842 | 5824 | 6200 | 5988 | 160.58 | 2.6% |
Protein Target Info & Infographic
Gene/Protein Information For CRTAC1 (Source: Uniprot.Org, NCBI)
Gene Name
CRTAC1
Full Name
Cartilage acidic protein 1
Weight
71.421kDa
Alternative Names
ASPIC; ASPIC1; ASPIC1CEP-68acidic secreted protein in cartilage; cartilage acidic protein 1; CEP68; chondrocyte expressed protein 68 kDa CEP-68,68 kDa chondrocyte-expressed protein; CRTAC1; FLJ10320 CRTAC1 ASPIC, ASPIC1, CEP-68, CEP68, LOTUS cartilage acidic protein 1 cartilage acidic protein 1|acidic secreted protein in cartilage|chondrocyte expressed protein 68 kDa CEP-68
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CRTAC1, check out the CRTAC1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CRTAC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CRTAC1 ELISA Kit PicoKine® (EK2088)
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No publications found for EK2088
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Human CRTAC1 ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for CRTAC1 ELISA kit? I used your CRTAC1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CRTAC1 even though the O.D. values are not very high?
M. Martinez
Verified customer
Asked: 2020-11-12
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CRTAC1 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of CRTAC1 that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2020-11-12
Question
Q: is it okay to use EDTA plasma as samples in Human CRTAC1 Picokine® ELISA Kit (Catalog # EK2088)?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CRTAC1 causing disruption of its protein structure. CRTAC1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CRTAC1 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-05-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-09-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-09-25
Question
Q: Can CRTAC1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
W. Phillips
Verified customer
Asked: 2017-12-11
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2017-12-11
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CRTAC1 using the EK2088 Human CRTAC1 Picokine® ELISA Kit?
P. Zhao
Verified customer
Asked: 2014-11-04
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2014-11-04
