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Product Info Summary
| SKU: | EK2196 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human ESAM ELISA Kit PicoKine®
SKU/Catalog Number
EK2196
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human ESAM ELISA Kit PicoKine™ (96 Tests). Quantitate Human ESAM in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ESAM ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2196)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q30-A247
Sensitivity
<20 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2196 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2196 is reactive to Esam in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2196 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Esam
Endothelial cell-selective adhesion molecule is a protein that in humans is encoded by the ESAM gene. Enables cell-cell adhesion mediator activity. Involved in several processes, including bicellular tight junction assembly; cell-cell adhesion; and regulation of actin cytoskeleton organization. Located in cell-cell junction and plasma membrane. Part of protein-containing complex.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2196-CAP | Anti-Human Esam Pre-coated 96-well strip microplate | 1 |
| EK2196-ST | Human Esam Standard | 2 vials, 20 ng/tube |
| EK2196-DA | Human Esam Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.025 | 0.054 | 0.088 | 0.152 | 0.369 | 0.727 | 1.263 | 1.965 |
Data Example Images
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Human ESAM PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ESAM ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 568 | 2236 | 7899 | 672 | 2115 | 7567 |
| Standard deviation | 33.51 | 114.04 | 458.14 | 41.66 | 137.48 | 336.91 |
| CV(%) | 5.9% | 5.1% | 5.8% | 6.2% | 6.5% | 4.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Esam in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 568 | 557 | 562 | 514 | 550 | 26.96 | 4.9% |
| Sample 2 | 2236 | 2048 | 2154 | 2092 | 2133 | 95.96 | 4.5% |
| Sample 3 | 7899 | 7713 | 7541 | 8136 | 7822 | 391.11 | 5% |
Protein Target Info & Infographic
Gene/Protein Information For Esam (Source: Uniprot.Org, NCBI)
Gene Name
Esam
Full Name
Endothelial cell-selective adhesion molecule
Weight
41.81kDa
Alternative Names
2310008D05Rik; endothelial cell adhesion molecule; endothelial cell-selective adhesion molecule; ESAM; HUEL (C4orf1)-interacting protein; LP4791 protein; W117m Esam|2310008D05Rik1, W117m, Esam|endothelial cell-specific adhesion molecule|endothelial cell-selective adhesion molecule
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Esam, check out the Esam Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Esam: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human ESAM ELISA Kit PicoKine® (EK2196)
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