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Product Info Summary
| SKU: | EK2201 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human FGL1 ELISA Kit PicoKine®
View all Hepassocin/FGL1 ELISA kits
SKU/Catalog Number
EK2201
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human FGL1 Kit PicoKine™ (96 Tests). Quantitate Human FGL1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FGL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2201)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L23-I312
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2201 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2201 is reactive to FGL1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2201 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Hepassocin/FGL1
Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FGL-1 is encoded by the FGL1 gene. Fibrinogen-like 1 is a member of the fibrinogen family. This protein is homologous to the carboxy terminus of the fibrinogen beta- and gamma- subunits which contains the four conserved cysteines of fibrinogens and fibrinogen related proteins. However, this protein lacks the platelet-binding site, cross-linking region and a thrombin-sensitive site which are necessary for fibrin clot formation. This protein may play a role in the development of hepatocellular carcinomas. Four alternatively spliced transcript variants encoding the same protein exist for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2201-CAP | Anti-Human FGL1 Pre-coated 96-well strip microplate | 1 |
| EK2201-ST | Human FGL1 Standard | 2 vials, 20 ng/tube |
| EK2201-DA | Human FGL1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.096 | 0.220 | 0.286 | 0.505 | 0.814 | 1.078 | 1.735 | 2.013 |
Data Example Images
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Human FGL1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FGL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 403 | 665 | 1490 | 507 | 779 | 1865 |
| Standard deviation | 12.90 | 27.27 | 56.62 | 28.39 | 48.30 | 87.66 |
| CV(%) | 3.2% | 4.1% | 3.8% | 5.6% | 6.2% | 4.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Hepassocin/FGL1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 403 | 462 | 502 | 485 | 463 | 23.61 | 5.1% |
| Sample 2 | 665 | 725 | 776 | 685 | 713 | 37.06 | 5.2% |
| Sample 3 | 1490 | 1645 | 1550 | 1516 | 1550 | 74.41 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For FGL1 (Source: Uniprot.Org, NCBI)
Gene Name
FGL1
Full Name
Fibrinogen-like protein 1
Weight
36.379kDa
Alternative Names
FGL1; fibrinogen-like 1; fibrinogen-like protein 1; Fibrinogen-like-protein 1; FREP1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1MGC12455; HP-041; LFIRE1; LFIRE-1; Liver fibrinogen-related protein 1 FGL1 HFREP1, HP-041, HPS, LFIRE-1, LFIRE1 fibrinogen like 1 fibrinogen-like protein 1|hepassocin|hepatocellular carcinoma-related sequence|hepatocyte-derived fibrinogen-related protein 1|liver fibrinogen-related protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FGL1, check out the FGL1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FGL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human FGL1 ELISA Kit PicoKine® (EK2201)
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