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Product Info Summary
| SKU: | EK2127 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum |
Product info
Product Name
Human IFNA2 ELISA Kit PicoKine®
SKU/Catalog Number
EK2127
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IFNA2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IFNA2 in cell culture supernatants and serum. Sensitivity: 1 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IFNA2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2127)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: C24-E189
Sensitivity
<1 pg/ml
Assay Range
3.12 pg/ml - 200 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2127 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2127 is reactive to IFNA2 in Human samples
Validated Sample Types
cell culture supernatants and serum
Application Guarantee
EK2127 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IFNA2
Interferon alpha-2 is a protein that in humans is encoded by the IFNA2 gene. This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded cytokine is a member of the type I interferon family that is produced in response to viral infection as a key part of the innate immune response with potent antiviral, antiproliferative and immunomodulatory properties. This cytokine, like other type I interferons, binds a plasma membrane receptor made of IFNAR1 and IFNAR2 that is ubiquitously expressed, and thus is able to act on virtually all body cells. The encoded protein is effective in reducing the symptoms and duration of the common cold and in treating many types of cancer, including some hematological malignancies and solid tumors. A deficiency of type I interferon in the blood is thought to be a hallmark of severe COVID-19 and may provide a rationale for a combined therapeutic approach.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2127-CAP | Anti-Human IFNA2 Pre-coated 96-well strip microplate | 1 |
| EK2127-ST | Human IFNA2 Standard | 2 vials, 1 ng/tube |
| EK2127-DA | Human IFNA2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
| O.D. | 0.049 | 0.176 | 0.266 | 0.417 | 0.681 | 1.101 | 1.657 | 1.986 |
Data Example Images
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Human IFNA2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IFNA2, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 3.1 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IFNA2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 5 | 19 | 69 | 4 | 21 | 72 |
| Standard deviation | 0.21 | 0.82 | 2.69 | 0.20 | 1.24 | 4.10 |
| CV(%) | 4.2% | 4.3% | 3.9% | 5.1% | 5.9% | 5.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IFNA2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 5 | 4 | 6 | 5 | 5 | 0.11 | 2.2% |
| Sample 2 | 19 | 22 | 20 | 15 | 19 | 0.51 | 2.7% |
| Sample 3 | 69 | 71 | 75 | 80 | 74 | 2.58 | 3.5% |
Protein Target Info & Infographic
Gene/Protein Information For IFNA2 (Source: Uniprot.Org, NCBI)
Gene Name
IFNA2
Full Name
Interferon alpha-2
Weight
21.55kDa
Superfamily
alpha/beta interferon family
Alternative Names
IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D IFNA2 IFN-alpha-2, IFN-alphaA, IFNAB, leIF A, IFNA2 interferon alpha 2 interferon alpha-2|alpha-2a interferon|interferon alpha 2a|interferon alpha 2b|interferon alpha A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IFNA2, check out the IFNA2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IFNA2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IFNA2 ELISA Kit PicoKine® (EK2127)
Hello CJ!
EK2127 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A novel atherogenic epitope from Mycobacterium tuberculosis heat shock protein 65 enhances atherosclerosis in rabbit and LDL receptor-deficient mice
Singh A,Das D,Kurra S,Arava S,Gupta S,Sharma A.Dendritic cells and their associated pro-inflammatory cytokines augment to the inflammatory milieu in vitiligo skin.Cytokine.2021 Jun 5:155598.doi:10.1016/j.cyto.2021.155598.Epub ahead of print.PMID:34103210.
Species: Human
EK2127 usage in article: APP:ELISA, SAMPLE:DENDRITIC CELL, DILUTION:NA
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