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Product Info Summary
| SKU: | EK2226 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human IL10RB ELISA Kit PicoKine®
View all IL-10 R beta ELISA kits
SKU/Catalog Number
EK2226
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL10RB PicoKine ELISA Kit® (96 Tests). Quantitate Human IL10RB in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL10RB ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2226)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: M20-S220
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2226 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2226 is reactive to IL10RB in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2226 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-10 R beta
Interleukin 10 receptor, beta subunit is a subunit for the interleukin-10 receptor. IL10RB is its human gene. The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2226-CAP | Anti-Human IL10RB Pre-coated 96-well strip microplate | 1 |
| EK2226-ST | Human IL10RB Standard | 2 vials, 10 ng/tube |
| EK2226-DA | Human IL10RB Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.026 | 0.052 | 0.133 | 0.365 | 0.714 | 1.121 | 1.497 | 2.058 |
Data Example Images
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Human IL10RB PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL10RB ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 224 | 359 | 4418 | 173 | 561 | 3368 |
| Standard deviation | 7.86 | 18.52 | 341.51 | 11.71 | 37.19 | 264.1 |
| CV(%) | 3.5% | 5.2% | 7.7% | 6.8% | 6.6% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-10 R beta in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 224 | 259 | 297 | 189 | 242 | 14.46 | 6% |
| Sample 2 | 359 | 577 | 478 | 517 | 483 | 37.46 | 7.8% |
| Sample 3 | 4418 | 2596 | 4568 | 2765 | 3587 | 258.96 | 7.2% |
Protein Target Info & Infographic
Gene/Protein Information For IL10RB (Source: Uniprot.Org, NCBI)
Gene Name
IL10RB
Full Name
Interleukin-10 receptor subunit beta
Weight
36.995kDa
Superfamily
type II cytokine receptor family
Alternative Names
CDw210b antigen; CDw210b; CRF2-4IL-10R subunit 2; CRFB4; CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B; D21S58; D21S66; IBD25; IL-10 R beta; IL-10 receptor subunit beta; IL10R beta; IL-10R2; IL-10R2IL-10R subunit beta; IL10RB; IL-10Rb; interleukin 10 receptor, beta; Interleukin-10 receptor subunit 2; interleukin-10 receptor subunit beta; member 4 IL10RB CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2 interleukin 10 receptor subunit beta interleukin-10 receptor subunit beta|IL-10 receptor subunit beta|IL-10R subunit 2|IL-10R subunit beta|IL-10RB|cytokine receptor class-II CRF2-4|cytokine receptor class-II member 4|cytokine receptor family 2 member 4|cytokine receptor family II, member 4|interleukin 10 receptor, beta|interleukin-10 receptor subunit 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL10RB, check out the IL10RB Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL10RB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL10RB ELISA Kit PicoKine® (EK2226)
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