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Product Info Summary
| SKU: | EK2233 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human LRG1 ELISA Kit PicoKine®
SKU/Catalog Number
EK2233
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human LRG1 PicoKine ELISA Kit® (96 Tests). Quantitate Human LRG1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 300 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LRG1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2233)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: HEK293; Immunogen sequence: V36-Q347
Sensitivity
<300 pg/ml
Assay Range
625 pg/ml - 40,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2233 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2233 is reactive to LRG1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2233 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of LRG1
Leucine-rich alpha-2-glycoprotein 1 is a protein which in humans is encoded by the gene LRG1. The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2233-CAP | Anti-Human LRG1 Pre-coated 96-well strip microplate | 1 |
| EK2233-ST | Human LRG1 Standard | 2 vials, 40 ng/tube |
| EK2233-DA | Human LRG1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
| O.D. | 0.017 | 0.129 | 0.212 | 0.335 | 0.640 | 0.961 | 1.472 | 2.084 |
Data Example Images
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Human LRG1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LRG1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1060 | 2321 | 18753 | 626 | 1268 | 12203 |
| Standard deviation | 42.29 | 118.84 | 1177.69 | 14.27 | 91.42 | 541.81 |
| CV(%) | 4% | 5.1% | 6.3% | 2.3% | 7.2% | 4.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of LRG1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1060 | 820 | 1217 | 1160 | 1064 | 60.77 | 5.7% |
| Sample 2 | 2321 | 1411 | 1653 | 1516 | 1725 | 113.18 | 6.6% |
| Sample 3 | 18753 | 15634 | 16014 | 17741 | 17036 | 1333.88 | 7.8% |
Protein Target Info & Infographic
Gene/Protein Information For LRG1 (Source: Uniprot.Org, NCBI)
Gene Name
LRG1
Full Name
Leucine-rich alpha-2-glycoprotein
Weight
38.178kDa
Alternative Names
1300008B03Rik; 2310031E04Rik; HMFT1766; leucine-rich alpha-2-glycoprotein 1; leucine-rich alpha-2-glycoprotein; LRG1; LRGleucine rich alpha 2 glycoprotein LRG1 HMFT1766, LRG leucine rich alpha-2-glycoprotein 1 leucine-rich alpha-2-glycoprotein|1300008B03Rik|2310031E04Rik|leucine rich alpha 2 glycoprotein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LRG1, check out the LRG1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LRG1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human LRG1 ELISA Kit PicoKine® (EK2233)
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