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Product Info Summary
| SKU: | EK2274 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human MFAP5 ELISA Kit PicoKine®
View all MAGP-2/MFAP5 ELISA kits
SKU/Catalog Number
EK2274
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MFAP5 ELISA Kit PicoKine® (96 Tests). Quantitate Human MFAP5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 75 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MFAP5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2274)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: I22-L173
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2274 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2274 is reactive to MFAP5 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2274 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MAGP-2/MFAP5
Microfibrillar-associated protein 5 is a protein that in humans is encoded by the MFAP5 gene. This gene encodes a 25-kD microfibril-associated glycoprotein which is a component of microfibrils of the extracellular matrix. The encoded protein promotes attachment of cells to microfibrils via alpha-V-beta-3 integrin. Deficiency of this gene in mice results in neutropenia. Alternate splicing results in multiple transcript variants encoding different isoforms.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2274-CAP | Anti-Human MFAP5 Pre-coated 96-well strip microplate | 1 |
| EK2274-ST | Human MFAP5 Standard | 2 vials, 10 ng/tube |
| EK2274-DA | Human MFAP5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.042 | 0.141 | 0.252 | 0.461 | 0.636 | 0.997 | 1.371 | 2.046 |
Data Example Images
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Human MFAP5 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MFAP5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 306 | 393 | 3203 | 267 | 394 | 3107 |
| Standard deviation | 7.47 | 13.32 | 94.49 | 14.85 | 14.38 | 122.73 |
| CV(%) | 2.4% | 3.4% | 3% | 5.6% | 3.7% | 4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MAGP-2/MFAP5 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 306 | 178 | 273 | 175 | 233 | 14.17 | 6.1% |
| Sample 2 | 393 | 415 | 409 | 410 | 407 | 24.57 | 6% |
| Sample 3 | 3203 | 4322 | 4523 | 4416 | 4116 | 179.05 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For MFAP5 (Source: Uniprot.Org, NCBI)
Gene Name
MFAP5
Full Name
Microfibrillar-associated protein 5
Weight
19.612kDa
Superfamily
MFAP family
Alternative Names
MAGP2; MAGP-2; MAGP2MFAP-5; MFAP5; Microfibril-associated glycoprotein 2; microfibrillar associated protein 5; microfibrillar-associated protein 5; MP25; MP25microfibril-associated glycoprotein-2 MFAP5 AAT9, MAGP-2, MAGP2, MFAP-5, MP25 microfibril associated protein 5 microfibrillar-associated protein 5|THE1A-MFAP5|microfibril-associated glycoprotein-2|microfibrillar associated protein 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MFAP5, check out the MFAP5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MFAP5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MFAP5 ELISA Kit PicoKine® (EK2274)
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